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Establishment and characterization of a cell line from the american opossum (Didelphys virginiana)

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Summary

A permanent tissue-cultured cell line (designated OK) has been established from kidney tissue of an adult American opossum. The OK line has been characterized with respect to morphology, chromosome constitution, tissue-culture requirements, and attainable mitotic arrest. The cells are epithelial-like with a stable nondiploid chromosomal modal number of 23. Cells grown in Eagle's minimal essential medium with 10% fetal bovine serum have a mean doubling time of 18 hr. The cell line OK is potentially useful for the isolation and purification of the mammalian X chromosome because of the size differential between the smaller X's and the larger autosomes.

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Author notes

  1. H. Koyama

    Present address: Cancer Institute, Japanese Foundation for Cancer Research, Kami-Ikebukuro, Toshima-Ku, 170, Tokyo, Japan

  2. C. Goodpasture

    Present address: Metabolism and Radiation Research Laboratory, North Dakota State University, 58102, Fargo, North Dakota

Authors and Affiliations

  1. Department of Biology and Department of Cytogenetics and Cytology, City of Hope National Medical Center, 1500 East Duarte Road, 91010, Duarte, California

    H. Koyama, C. Goodpasture, M. M. Miller, R. L. Teplitz & A. D. Riggs

Authors
  1. H. Koyama
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  2. C. Goodpasture
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  3. M. M. Miller
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  4. R. L. Teplitz
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  5. A. D. Riggs
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Additional information

This work was supported by NIH grant HD-04420.

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Koyama, H., Goodpasture, C., Miller, M.M. et al. Establishment and characterization of a cell line from the american opossum (Didelphys virginiana). In Vitro 14, 239–246 (1978). https://doi.org/10.1007/BF02616032

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