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Growth characteristics of bone marrow cells from beige mutant, the mouse homologue of the Chediak-Highshi syndrome of man, propagated in semisolid agar cultures

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Summary

Suspensions of bone marrow cells from the beige (bg/bg) mouse, a homologue of the Chediak-Higashi syndrome (C-HS) of man, and normal mouse bone marrow cells, when stimulated by colony-stimulating factor (CSF) from different sources, proliferate in semisolid agar cultures and produce colonies composed of granulocytic and/or mononuclear cells. Studies with CSF from various sources (embryo and kidney feeder monolayers, conditioned media from and kidney cell cultures, and plasma from untreated, irradiated, or endotoxin-treated mice) indicated that bone marrow cells mal cells when the incubated with the same CSF source. Experiments which compared the bone marrow colony response of beige and normal cells using postendotoxin plsma (PEP) as the CSF source indicated that beige cells were stimulated to the same extent by CSF obtained from either the normal or the beige mouse. CSF obtained from normal or affected mice was equally effective in stimulating bone marrow cell proliferation. There was no discordance of colony cell types present when PEP-stimulated 8-day bone marrow colonies from normal and affected mice were compared. The nuclear morphology of beige and normal granulocytes from these cultures indicated cells ranging from myeloblasts to mature segmented polymorphonuclear leukocytes. These preliminary studies indicate that the agar culture method for the growth of mono-nuclear and granulocytic colonies may provide a method for obtaining enriched C-HS cell populations for biochemical analysis of the genetic efect(s) in this interesting disease.

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Authors and Affiliations

  1. Department of Veterinary Microbiology and Pathology, Washington State University, 99163, Pullman, Washington

    Harland W. Renshaw D.V.M., Ph.D. & William C. Davis

  2. Pioneering Research Laboratory Western Region, Agricultural Research Service, U. S. Department of Agriculture, 99163, Pullman, Washington

    Harland W. Renshaw D.V.M., Ph.D. & William C. Davis

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  1. Harland W. Renshaw D.V.M., Ph.D.
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  2. William C. Davis
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Additional information

This investigation was supported in part by USPHS grants AI-06591, AI-10290, and General Research Support FR 5465.

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Renshaw, H.W., Davis, W.C. Growth characteristics of bone marrow cells from beige mutant, the mouse homologue of the Chediak-Highshi syndrome of man, propagated in semisolid agar cultures. In Vitro 11, 5–13 (1975). https://doi.org/10.1007/BF02615316

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