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Isolation of lysine codon suppressors inEscherichia coli

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Abstract

Starting with anEscherichia coli strain containingglyT56, a glycine transfer RNA suppressor of the arginine codons AGA and AGG, and atrpA mutant containing lysine at position 211 of the tryptophan synthetase alpha chain, we have isolated AAG-suppressors that fall into two classes. In class 1 are dominant suppressors that arose with the simultaneous loss ofglyT56 activity. They are approximately 50% cotransducible withargE, as isglyT, and appear to be derived fromglyT56. Class 2 suppressors, located betweenpurE andtrp on theE. coli map, are not near any glycine tRNA genes, and may represent novel missense suppressors.

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Authors and Affiliations

  1. Department of Biology, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, 77030, Houston, Texas, USA

    Kathleen H. Hadley & Emanuel J. Murgola

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  1. Kathleen H. Hadley
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  2. Emanuel J. Murgola
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Hadley, K.H., Murgola, E.J. Isolation of lysine codon suppressors inEscherichia coli . Current Microbiology 1, 99–103 (1978). https://doi.org/10.1007/BF02605425

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