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Patterns of wall synthesis inSaccharomyces cerevisiae

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Abstract

Wall formation inSaccharomyces cerevisiae seems to be the result of two main patterns of wall material deposition: (i) around the whole periphery of the cell in nonbudding ones, and (ii) mainly at the tip of the daughter cell or at the cross wall that separates dividing cells. This interpretation has been obtained following experiments in which RNA or protein synthesis has been inhibited. Under these conditions, glucan formation takes place, and wall thickening is probably due to the accumulation of this polysaccharide. Furthermore, once a pattern of wall deposition has been established, it is not modified by inhibition of RNA or protein synthesis.

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Literature Cited

  1. Bálint, S., Farkas, V., Bauer, S. 1976. Biosynthesis of β-glucans catalyzed by a particulate enzyme preparation from yeast. FEBS Letters64:44–47.

    Article  PubMed  Google Scholar 

  2. Beteta, P., Gascón, S. 1971. Localization of invertase in yeast vacuoles. FEBS Letters13:297–300.

    Article  PubMed  CAS  Google Scholar 

  3. Biely, P., Kovarik, J., Bauer, S. 1973. Cell wall formation in yeast. An electron microscopic autoradiographic study. Archiv für Mikrobiologie94:365–371.

    Article  Google Scholar 

  4. Boer, P., Van Rijn, H. J. M., Reinking, A., Steyn-Parvé, E. P. 1975. Biosynthesis of acid phosphatase of baker’s yeast. Characterization of a protoplast bound fraction containing precursors of the exoenzyme. Biochemica et Biophysica Acta377:331–342.

    CAS  Google Scholar 

  5. Cortat, M., Matile, Ph., Kopp, F. 1973. Intracellular localization of mannan synthetase activity in budding baker’s yeast. Biochemical and Biophysical Research Communications53:482–489.

    Article  PubMed  CAS  Google Scholar 

  6. Cortat, M., Wiemken, A. 1972. Isolation of glucanase-containing vesicles from budding yeast. Archiv für Mikrobiologie82:189–205.

    Article  PubMed  CAS  Google Scholar 

  7. Dominguez, A., Villanueva, J. R., Sentandreu, R. 1978. Inositol deficiency inSaccharomyces cerevisiae NCYC 86. Antonie van Leeuwenhoek Journal of Microbiology and Serology44:25–34.

    Article  CAS  Google Scholar 

  8. Durán, A., Bowers, B., Cabib, E. 1975. Chitin synthetase zymogen is attached to the yeast plasma membrane. Proceedings of the National Academy of Sciences of the United States of America10:3952–3955.

    Article  Google Scholar 

  9. Elorza, M. V., Larriba, G., Villanueva, J. R., Sentandreu, R. 1977. Transactions of the British Mycological Society69:451–457.

    Article  Google Scholar 

  10. Elorza, M. V., Lostau, C. M., Villanueva, J., Sentandreu, R. 1976. Cell wall synthesis regulation inSaccharomyces cerevisiae. Effect of RNA and protein synthesis inhibition. Biochimica et Biophysica Acta454:263–272.

    PubMed  CAS  Google Scholar 

  11. Hutchison, H. T., Hartwell, L. H., McLaughlin, C. S. 1969. Temperature sensitive yeast mutant defective in ribonucleic acid production. Journal of Bacteriology99:807–814.

    PubMed  CAS  Google Scholar 

  12. Johnson, B. J., Gibson, E. J. 1966. Autoradiographic analysis of regional cell wall growth of yeasts. III.Saccharomyces cerevisiae. Experimental Cell Research,41:580–591.

    Article  PubMed  CAS  Google Scholar 

  13. Kosinová, A., Farkás, V., Machala, S., Bauer, S. 1974. Site of mannan synthesis in yeast. An autoradiographic study. Archiv für Mikrobiologie99:255–263.

    Google Scholar 

  14. Lampen, J. O., Kuo, S. C., Cano, F. R., Tkacz, J. S. 1972. Structural features in synthesis of external enzymes by yeast, pp. 819–824. In: Terui, G. (ed.), Fermentation Technology Today: Proceedings of the IVth International Fermentation Symposium. Osaka, Japan: Society of Fermentation Technology.

    Google Scholar 

  15. Linnemans, W. A. M., Boer, P., Elbers, P. F. 1977. Localization of acid phosphatase inSaccharomyces cerevisiae: A clue to cell wall formation. Journal of Bacteriology131:638–644.

    PubMed  CAS  Google Scholar 

  16. López-Romero, E., Ruiz-Herrera, J. 1977. Biosynthesis of β-glucans by cell-free extracts fromSaccharomyces cerevisiae. Biochimica et Biophysica Acta500:372–384.

    PubMed  Google Scholar 

  17. Meyer, J., Matile, Ph. 1975. Subcellular distribution of yeast invertase isoenzymes. Archives of Microbiology103:51–55.

    Article  PubMed  CAS  Google Scholar 

  18. Moor, H. 1967. Endoplasmic reticulum as the initiator of bud formation in yeasts. Archiv für Mikrobiologie57:135–146.

    Article  PubMed  CAS  Google Scholar 

  19. Necas, O. 1971. Cell wall synthesis in yeast protoplasts. Bacteriological Reviews35:149–170.

    PubMed  CAS  Google Scholar 

  20. Reynolds, E. S. 1963. The use of lead citrate at high pH as an electron opaque stain in electron microscopy. Journal of Cell Biology17:208–212.

    Article  PubMed  CAS  Google Scholar 

  21. Santos, E., Villanueva, J. R., Sentandreu, R. 1978. The plasma membrane ofSaccharomyces cerevisiae. Isolation and some properties. Biochimica et Biophysica Acta508:39–54.

    Article  PubMed  CAS  Google Scholar 

  22. Sentandreu, R., Elorza, M. V., Villanueva, J. R. 1975. Synthesis of yeast wall glucan. Journal of General Microbiology90:13–20.

    PubMed  CAS  Google Scholar 

  23. Sentandreu, R., Northcote, D. H. 1969. The formation of buds in yeast. Journal of General Microbiology55:393–398.

    PubMed  CAS  Google Scholar 

  24. Spurr, A. R. 1969. A low viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research26:31–43.

    Article  PubMed  CAS  Google Scholar 

  25. Williams, R. J., Eakin, R. E., Snell, E. F. 1940. The relationship of inositol, thiamin, biotin, pantothenic acid and vitamin B6 to the growth of yeasts. Journal of the American Chemical Society62:1204–1207.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Departamento de Microbiología, Facultad de Farmacia, Universidad de Valencia, Avda. Blasco Ibañez, 13, 10, Valencia, Spain

    Luis Rodriguez & Rafael Sentandreu

  2. Departamento de Microbiología, Facultad de Farmacia, Universidad de Alcalá de Henares, Spain

    Fernando Laborda

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  1. Luis Rodriguez
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  2. Fernando Laborda
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  3. Rafael Sentandreu
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Rodriguez, L., Laborda, F. & Sentandreu, R. Patterns of wall synthesis inSaccharomyces cerevisiae . Current Microbiology 2, 293–297 (1979). https://doi.org/10.1007/BF02602862

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