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Genomic structure, chromosome location, and alternative splicing of the humanNKG2A gene

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Abstract

A cosmid containing the humanNKG2A gene was isolated. The gene was partially sequenced, revealing 7 exons, including one 5′ untranslated exon. The 54 base pair exon 5 was missing in the published cDNA cloneNKG2B, consistent with the existence of differential splicing. This was confirmed by reverse transcription-polymerase chain reaction of total RNA from normal lymphocytes. TheNKG2A gene was mapped by FISH to chromosome 12p12.3-p13.1 in proximity to theCD69 andPrp genes. These data support the presence of a human lectin-likeNK gene complex, analogous to theNK complex on mouse chromosome 6.

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Authors and Affiliations

  1. Human Immunogenetics Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, WC2A 3PX, London, England

    Béatrice Plougastel & John Trowsdale

  2. Human Cytogenetics Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, WC2A 3PX, London, England

    Tania Jones

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  1. Béatrice Plougastel
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  2. Tania Jones
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Plougastel, B., Jones, T. & Trowsdale, J. Genomic structure, chromosome location, and alternative splicing of the humanNKG2A gene. Immunogenetics 44, 286–291 (1996). https://doi.org/10.1007/BF02602558

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  • DOI: https://doi.org/10.1007/BF02602558

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