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Growth ofNeisseria gonorrhoeae in media deficient in iron without detection of siderophores

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Abstract

A disseminating strain ofNeisseria gonorrhoeae was grown in a liquid, defined medium containing different levels of a strong chelator of ferric iron, ethylenediamine-di (o-hydroxyphenyl acetic acid) (EDDA). The inhibition of growth produced by EDDA was relieved by addition of iron. Filtrates from cultures grown in the media deficient in iron were examined for presence of siderophores, microbial chelators of iron, by colorimetric tests for phenolates and hydroxamates and by two biological assays in which the filtrates were tested for ability to stimulate growth of small inocula and for ability to prevent bactericidal effects of normal human serum. By use of these methods, none of the filtrates displayed evidence of gonococcal siderophores.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Baylor College of Medicine, 77030, Houston, Texas, USA

    Pinina Norrod & Robert P. Williams

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  1. Pinina Norrod
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  2. Robert P. Williams
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Norrod, P., Williams, R.P. Growth ofNeisseria gonorrhoeae in media deficient in iron without detection of siderophores. Current Microbiology 1, 281–284 (1978). https://doi.org/10.1007/BF02601682

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