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A thermophilic glucoamylase fromCephalosporium eichhorniae

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Abstract

A novel exocellular glucoamylase produced by a thermophilic fungus,Cephalosporium eichhorniae, was purified by a combination of membrane filtration and Sephadex chromatography. The enzyme was a glycoprotein, 28% carbohydrate by weight. It was composed of a single polypeptide chain with a molecular weight of 26,850. The enzyme was thermostable with optimum activity between 45 and 62°C. It had a substrate preference of amylose>amylopectin. Analysis by thin-layer and gas-liquid chromatography showed the major hydrolytic product of starch was glucose, classifying this enzyme as a thermophilic glucoamylase.

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  1. Department of Microbiology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada

    D. F. Day

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  1. D. F. Day
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Day, D.F. A thermophilic glucoamylase fromCephalosporium eichhorniae . Current Microbiology 1, 181–184 (1978). https://doi.org/10.1007/BF02601674

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