Abstract
Intralipid is a light scattering medium used in photodynamic therapy (PDT) in both phantom studies and to obtain optimum light distributions from clinical light delivery systems. Light delivery to a target is one variable in PDT over which we have direct control, and should be as accurate as possible. Variations in the scattering ability of Intralipid from batch to batch will lead to an unpredictable light distribution and undesirable PDT effects. Furthermore, 10% and 20% stock solutions, when diluted to similar final concentrations, have different scattering abilities which will lead to inaccuracies in studies measuring scattering coefficients and imprecision in light distributions from light delivery systems. Batch to batch variations of similar stock solutions should lead to minimal differences in outputs from light delivery systems. A simple and quick assay system is described to define the concentration of Intralipid based on its light scattering ability, which enables testing of solutions prior to use. In the clinical setting where accurate light distribution is required, we suggest that only one stock solution (e.g. 20%) is used and that the final dilution scattering ability is checked using this assay system.
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References
Wilson BC, Patterson MS, Burns DM. Effect of photosensitizer concentration in tissue on the penetration depth of photoactivating light.Laser Med Sci 1986,1:235–44
Flock ST, Wilson BC, Patterson MS. Total attenuation coefficients and scattering phase functions of tissues and phantom materials at 633 nm.Med Phys 1987,14:835–41
Jocham D, Staehler G, Chaussy Ch et al. Integral dye laser irradiation of photosensitised bladder tumours with the aid of a light scattering medium.Prog Clin Biol Res 1984,170:249–56
Baghdassarian R, Wright MW, Vaughan SA et al. The use of lipid emulsion as an intravesical medium to disperse light in the potential treatment of bladder tumors.J Urol 1985,133:126–30
Muller PJ, Wilson BC. Photodynamic therapy: cavitary photoillumination of malignant cerebral tumours using a laser coupled inflatable balloon.Can J Neurol Sci 1985,12:371–3
Wilson BC, Muller PJ, Yanch JC. Instrumentation and light dosimetry for intraoperative photodynamic therapy (PDT) of malignant brain tumours.Phys Med Biol 1986,31:125–33
Driver I, Feather JW, King PR, Dawson JB. The optical properties of aqueous suspensions of Intralipid, a fat emulsion.Phys Med Biol 1989,34:1927–30
Lindley EJ. Report of the photodynamic therapy dosimetry meeting: April 1989.Lasers Med Sci 1989,4:211–20
Dahlman A, Wile AG, Burns RG et al. Laser photoradiation therapy of cancer.Cancer Res 1983,43:430–4
Spikes JD, Jori G. Photodynamic therapy of tumors and other diseases using porphyrins.Lasers Med Sci 1987,2:3–15
Van Staveren HJ, Moes CJM, Van Marle J et al. Light scattering in Intralipid 10% in the wavelength range of 400–1100 nm.Appl Opt 1991,30:4507–14
Boylan JV. Essential elements of quality control.Am J Hosp Pharm 1983,40:1936–9
Allardice JT, Abulafi AM, Dean R, Rowland AC, Williams NS. Light delivery systems for adjunctive intraoperative photodynamic therapy.Lasers Med Sci (in press).
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Allardice, J.T., Abulafi, A.M., Webb, D.G. et al. Standardization of intralipid for light scattering in clinical photodynamic therapy. Laser Med Sci 7, 461–465 (1992). https://doi.org/10.1007/BF02594089
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DOI: https://doi.org/10.1007/BF02594089