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Asparaginase decreases clotting factors in vitro: a possible pitfall?

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International Journal of Clinical and Laboratory Research

Summary

l-Asparaginase treatment of leukemia patients causes hemostatic problems. To investigate whetherl-asparaginase influences coagulation studies, 63 blood samples of 21 healthy male donors were incubated withl-asparaginase for 30 min at room temperature. After treatment with 100 IU/mll-asparaginase plasma fibrinogen (P=0.002), plasma antithrombin (P=0.0002), plasma protein C (P=0.0004), and plasma plasminogen (P=0.0039) were decreased compared with controls. In contrast, a significant increase in plasma von Willebrand factor antigen (P=0.08) and plasma thromboglobulin (P=0.005) was observed. The decrease in plasma antithrombin (P=0.001), plasma protein C (P=0.0003), and plasma plasminogen (P=0.0043) was also measurable after 0.05 IU/ml asparaginase treatment. The incubation withl-asparaginase was similar to the normal time from blood sampling to testing and hence the results suggest thatl-asparaginase may directly attack proteins of the coagulation system during the interval between sampling and assay.

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Authors and Affiliations

  1. Department of Pediatrics, Pediatric Hematology and Oncology, Westfälische Wilhelms-Universität, Albert-Schweitzer-Strasse 33, D-48149, Münster, Germany

    U. Nowak-Göttl, J. Boos, J. E. A. Wolff, H. Veltmann, G. Werber, E. Ahlke & H. Jürgens

  2. Boehringer-Mannheim, Germany

    H. Lill

Authors
  1. U. Nowak-Göttl
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  2. J. Boos
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  3. J. E. A. Wolff
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  4. H. Lill
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  5. H. Veltmann
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  6. G. Werber
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  7. E. Ahlke
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  8. H. Jürgens
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Nowak-Göttl, U., Boos, J., Wolff, J.E.A. et al. Asparaginase decreases clotting factors in vitro: a possible pitfall?. Int J Clin Lab Res 25, 146–148 (1995). https://doi.org/10.1007/BF02592556

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  • DOI: https://doi.org/10.1007/BF02592556

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