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A thiourea-UV assay for total glucosinolate content in rapeseed meals

  • Technical
  • Published:
Journal of the American Oil Chemists’ Society

Abstract

A rapid and sensitive method for the determination of the total glucosinolate content in rapeseed is described. The method is based on the specific UV absorbance of the thioureas and oxazolidine-2-thiones. Results obtained were confirmed by gas-liquid chromatography. Recoveries varying from 94 to 103% were obtained for samples containing mixtures of isothiocyanate and oxazolidine-2-thione (a total of 0.25 to 0.78 mg per assay). The relative stadard deviation for rapeseed meals varied from 4 to 10% for the total glucosinolate content (expressed as 3-butenylisothiocyanate) and depended on the size of sample taken. The relative standard deviation for oxazolidine-2-thione varied from 5 to 35% for the same meal. The lower limit of detection for rapeseed meal is of the order of 0.25 mg of 3-butenylisothiocyanate per g.

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Authors and Affiliations

  1. Prairie Regional Laboratory, National Research Council of Canada, S7N OW9, Saskatoon, Saskatchewan, Canada

    L. R. Wetter & C. G. Youngs

Authors
  1. L. R. Wetter
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  2. C. G. Youngs
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NRCC No. 15090.

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Wetter, L.R., Youngs, C.G. A thiourea-UV assay for total glucosinolate content in rapeseed meals. J Am Oil Chem Soc 53, 162–164 (1976). https://doi.org/10.1007/BF02586357

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  • DOI: https://doi.org/10.1007/BF02586357

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