Abstract
The role of oxygen free radicals (OFR) in the pathogenesis of many diseases is known. This experimental study was planned to investigate the role of OFR in acute abdominal inflammation. In this study, 20 adult rabbits were used. They were divided into two groups with 10 rabbits in each. In the study group, blood samples were taken from peripheral and mesenteric veins and then their appendices were ligated; after 24 h, blood samples were taken again and the appendices were resected. The same procedures were carried out in the control group, except for ligation of the appendices. Superoxide dismutase, catalase and glutathione peroxidase (GPX) activities were measured in all blood samples. The activities of catalase and GPX were increased postoperatively in peripheral and mesenteric blood samples in the experimental group. The catalase activity was increased in the control group. These results suggest that OFR increased sufficiently to activate the enzymatic defense system in acute appendicitis.
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References
Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assay and an assay applicable to acrylamide gels. Biochem J 44:276–287
Bulkley GB (1983) The role of oxygen free radicals in human disease processes. Surgery 94:407–411
Halliwell B, Gutteridge JMC (1984) Lipid peroxidation, oxygen radicals, cell damage and antioxidant therapy. Lancet II:1396–1397
Halliwell B, Gutteridge JMC (1984) Oxygen toxicity, Oxygen radicals, transition metals and disease. Biochem J 219:1–14
Köse K, Doĝan P (1992) Lipid peroksidasyonu (in Turkish). Erciyes Med J 1:340–350
Lelli JL, Pradhan S, Cobb M (1993) Prevention of postischemic injury in immature intestine by deferoxamine. J Surg Res 54:34–38
Mayes PA (1990) Biologic oxydation. In: Murray RK, Granner DK, Mayes PA, Rodwell VW (eds) Harper's Biochemistry. Appleton & Lange, Beirut, pp 105–111
McCord JM (1985) Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med 312:159–163
Otamiri T (1989) Oxygen radicals, lipid peroxidation, and neutrophil infiltration after small-intestinal ischemia and reperfusion. Surgery 105:593–597
Otamiri T, Tagesson C (1989) Role of phospholipase A2 and oxygenated free radicals in mucosal damage after small intestinal ischemia and reperfusion. Am J Surg 157:562–565
Paglia R, Valentina WN (1967) Studies on the quantitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169
Pigeolet E, Corbisier P, Houbion A, Lambert D, Michiels C, Raes M, Zachary MD, Remacle J (1990) Glutathion peroxidase, superoxide dismutase and catalase inactivation by peroxides and oxygen derived free radicals. Mech Ageing Dev 51:283–297
Reilly PM, Schilder HJ, Bulkley GB (1991) Pharmacologic approach to tissue injury mediated by free radicals and other reactive oxygen metabolites. Am J Surg 161:488–503
Rister M, Baehner RL (1976) The alteration of superoxide dismutase, catalase, glutathione peroxidase, and NAD(P)H Cytochrome c reductase in guinea pig polymorphonuclear leucocytes and alveolar macrophages during hyperoxia. J Clin Invest 58:1174–1184
Slater TF (1984) Overview of methods used for detecting lipid peroxidation. Methods Enzymol 105:283–293
Torii KK, Hayashi S, Nakamoto H et al (1982) Properties ofAspergillus niger catalase. J Biochem (Tokyo) 92:1449–1456
Wendel A (1988) Enzymes acting againts reactive oxygen. In: Goldberg DM, Moss DW, Schmidt E (eds) Enzymes—tools and targets. (Advances in clinical enzymology, vol 6). Karger, Basel, pp 161–167
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Turan, C., Küçükaydin, N., Doĝan, P. et al. The effect of acute ligation of the rabbit appendix on antioxidant enzymes. Res. Exp. Med. 196, 45–51 (1996). https://doi.org/10.1007/BF02576827
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DOI: https://doi.org/10.1007/BF02576827