Summary
The effects of equimolar concentrations (10−9 M) of ACTH and corticotropin-releasing hormone (CRH) on the secretory activity of zona glomerulosa (ZG) and zonae fasciculata and reticularis (ZF/ZR) of rat adrenals were investigated in vitro by high-pressure liquid chromatography. ACTH enhanced the output of all the post-progesterone steroids (11-deoxycorticosterone, 18-hydroxy-11-deoxycorticosterone, corticosterone, 18-hydroxycorticosterone, and aldosterone) both by isolated ZG or ZF/ZR cells and by adrenal slices. CRH raised the secretory activity exclusively of adrenal slices, and its effect was less than half that of ACTH. However, the extents to which the various post-progesterone hormones contributed to the ACTH- or CRH-induced rises in the overall adrenal secretory activity were similar. The hypothesis is discussed that CRH acts on the rat adrenal gland by eliciting a local production of ACTH.
Similar content being viewed by others
References
Aguilera G, Millan MA, Hauger RL, Catt KJ (1987) Corticotropin-releasing factor receptors: distribution and regulation in brain, pituitary and peripheral tissues. Ann NY Acad Sci 512:48–66
Andreis PG, Neri G, Cavallini L, Rebuffat P, Mazzocchi G, Nussdorfer GG (1989) Stereological and functional investigations on isolated adrenocortical cells. I. Adrenocortical cells from normal adult rats. J Submicrosc Cytol Pathol 21:257–265
Andreis PG, Neri G, Nussdorfer GG (1991) Corticotropin-releasing hormone (CRH) directly stimulates corticosterone secretion by the rat adrenal gland. Endocrinology 128:1998–2000
Belloni AS, Neri G, Musajo FG, Andreis PG, Boscaro M, D'Agostino D, Rebuffat P, Boshier DP, Gottardo G, Mazzocchi G, Nussdorfer GG (1990) Investigations on the morphology and function of adrenocortical tissue regenerated from gland capsular fragments autotransplanted in the musculus gracilis of the rat. Endocrinology 126:3251–3262
Bornstein SR, Ehrhart M, Scherbaum WA, Pfeiffer EF (1990) Adrenocortical atrophy of hypophysectomized rats can be reduced by corticotropin-releasings hormone (CRH). Cell Tissue Res 260:161–166
Buckingham JC (1987) A role for leukocytes in the control of adrenal steroidogenesis? J Endocrinol 114:1–2
Dave JR, Eiden LE, Eskay RL (1985) Corticotropin-releasings factor binding to peripheral tissue and activation of the adenylate cyclase-adenosine 3′,5′-monophosphate system. Endocrinology 116:2152–2159
Fehm HL, Holl R, Spatschwalbe E, Born J, Voight KH (1988) Ability of corticotropin releasing hormone to stimulate cortisol secretion independent from pituitary adrenocorticotropin. Life Sci 42:679–686
Gallo-Payet N, Pothier P, Isler H (1987) On the presence of chromaffin cells in the adrenal cortex: their possible role in adrenocortical function. Biochem Cell Biol 65:588–592
Hinson JP (1990) Paracrine control of adrenocortial function: a new role for the medulla? J Endocrinol 124:7–9
Jones CT, Edwards AV (1990) Release of adrenocorticotropin from the adrenal gland in the conscious calf. J Physiol (Lond) 426:397–407
Jones CT, Edwards, AV (1990) Adrenal responses to corticotrophin-releasing factor in conscious hypophysectomized calves. J Physiol (Lond) 430:25–36
Mazzocchi G, Rebuffat P, Meneghelli V, Nussdorfer GG (1989) Effects of the infusion with ACTH or CRH on the secretory activity of rat adrenal cortex. J Steroid Biochem 32:841–843
Nussdorfer GG (1986) Cytophysiology of the adrenal cortex. Int Rev Cytol 98:1–405
O'Hare MJ, Nice EC, Magee-Brown R, Bullman H (1976) High pressure liquid chromatography of steroids secreted by human adrenal and testis cells in monolayer cultur. J Chromatogr Biomed Appl 125:357–367
Smith EM, Morrill AC, Meyer WJ, Blalock JE (1986) Corticotropin releasing factor induction of leukocyte-derived immunoreactive ACTH and endorphin. Nature 321:881
Sparrow RA, Coupland RE (1987) Blood flow to the adrenal gland of the rat. J Anat 155:51–61
Suda T, Tomori N, Yajima F, Odagiri E, Demura H, Shizume K (1986) Characterization of immunoreactive corticotropin and corticotropin-releasing factor in human adrenal and ovarian tumors. Acta Endocrinol (Copenh), 111:546–552
Szalay KS (1981) Effect of pituitary intermediate lobe extract on steroid production by the isolated zona glomerulosa and fasciculata cells. Acta Physiol Acad Sci Hung 57:225–231
Tait JF, Tait SAS, Gould RP, Mee MSR (1974) The properties of adrenal zona glomerulosa cells after purification by gravitational sedimentation. Proc R Soc B 185:375–407
Vinson GP, Hinson JP, Raven PW (1985) The relationship between tissue preparation and function; methods for the study of control of aldosterone secretion: a review. Cell Biochem Funct 3:235–253
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Neri, G., Andreis, P.G. & Nussdorfer, G.G. Comparison of ACTH and corticotropin-releasing hormone effects on rat adrenal steriodogenesis in vitro. Res. Exp. Med. 191, 291–295 (1991). https://doi.org/10.1007/BF02576685
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02576685