Abstract
A prototype chamber was used to apply a precise cyclic or static load on articular cartilage explants under sterile conditions. A variable pressure, pneumatic controller was constructed to power the chamber's air cylinder, capable of applying, with a porous load platen, loads of up to 10 MPa at cycles ranging from 0 to 10 Hz. Pig articular cartilage explants were maintained successfully in this chamber for 2 days under cyclic mechanical loading of 0.5 Hz, 0.5 MPa. Explants remained sterile, viable and metabolically active. Cartilage responded to this load with a decreased synthesis of fibronectin and a small but statistically significant elevation in proteoglycan content. Similar but less extensive effects on fibronectin synthesis were observed with the small static load (0.016 MPa) inherent in the design of the chamber.
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References
Burton-Wurster N, Lust G (1985) Deposition of fibronectin in articular cartilage of canine osteroarthritic joints. Am J Vet Res 46:2542
Burton-Wurster N, Lust G (1990) Fibronectin and proteoglycan synthesis in long term cultures of cartilage explants in Ham's F12 supplemented with insulin and calcium: effects of the addition of TGF-beta. Arch Biochem Biophys 283:27
Engvall E, Ruoslathi E (1977) Binding of soluble form of fibroblast surface protein, fibronectin, to collagen. Int J Cancer 20:1
Farndale RW, Sayers CA, Barrett AJ (1982) A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures. Connect Tissue Res 9:247
Gray ML, Pizanelli AM, Grodzinsky AJ, Lee RC (1988) Mechanical and physicochemical determinants of the chondrocyte biosynthetic response. J Orthop Res 6:777
Hodge WA, Fijan RS, Carlson KG, Burgess RG, Harris WH, Mann RW (1986) Contact pressures in the human hip joint measured in vivo. Proc Natl Acad Sci USA 83:2879
Leipold HR, Goldberg RL, Lust G (1989) Canine serum keratan sulfate and hyaluronate concentration. Arthritis Rheum 32:312
Palmoski MJ, Brandt KD (1984) Effects of static and cyclic compressive loading on articular cartilage plugs in vitro. Arthritis Rheum 27:675
Parkkinen JJ, Lammi MJ, Karjalainen S, Laakkonen J, Hyvärinen E, Tihonen A, Helminen HJ, Tammi M (1989) A mechanical apparatus with microprocessor-controlled stress profile for cyclic compression of cultured articular cartilage explants. J Biomech 22:1285
Pena SDJ, Mills G, Hughes RC, Aptin JD (1980) Polypeptide heterogeneity of hamster and calf fibronectins. Biochem J 189:337
Sah RL-Y, Kim Y-J, Doong J-YH, Grodzinsky AJ, Plaas AHK, Sandy JD (1989) Biosynthetic response of cartilage explants to dynamic compression. J Orthop Res 7:619
Schneiderman R, Keret D, Maroudas A (1986) Effects of mechanical and osmotic pressure on the rate of glycosaminoglycan synthesis in the human adult femoral head cartilage: an in vitro study. J Orthop Res 4:393
Wurster NB, Lust G (1982) Fibronectin in canine osteoarthritic articular cartilage. Biochem Biophys Res Commun 109:1094
Wurster NB, Lust G (1984) Synthesis of fibronectin in normal and osteoarthritic articular cartilage. Biochim Biophys Acta 80:52
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Steinmeyer, J., Torzilli, P.A., Burton-Wurster, N. et al. A new pressure chamber to study the biosynthetic response of articular cartilage to mechanical loading. Res. Exp. Med. 193, 137–142 (1993). https://doi.org/10.1007/BF02576220
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DOI: https://doi.org/10.1007/BF02576220