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Histochemical properties of insularβ-cells after short tryptic digestion

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Summary

Aldehyde-fuchsin, without prior oxidation, was noted to stain the insularβ-cell granules of pancreas sections briefly pretreated with trypsin. Furthermore, after that procedure, insularβ-cells were more intensely basophilic than in control sections not previously subjected to tryptic digestion.

These findings are regarded as due to the appearance of new acidic groups within the insulin molecule, owing to the peculiar proteolytic activity of trypsin.

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References

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Authors and Affiliations

  1. Institute of Pathological Anatomy, University of Bologna, Bologna, Italy

    A. M. Mancini, G. Frizzera & A. Vecchi

Authors
  1. A. M. Mancini
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  2. G. Frizzera
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  3. A. Vecchi
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Mancini, A.M., Frizzera, G. & Vecchi, A. Histochemical properties of insularβ-cells after short tryptic digestion. Histochemie 6, 301–304 (1966). https://doi.org/10.1007/BF02570674

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  • DOI: https://doi.org/10.1007/BF02570674

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