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Increasing n-3 polyunsaturated fatty acid content of fish oil by temperature control of lipase-catalyzed acidolysis

  • Short Communication
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Journal of the American Oil Chemists’ Society

Abstract

An attempt was made to further increase the content of n-3 polyunsaturated fatty acid (n-3 PUFA) of fish oil by lipase-catalyzed acidolysis (reaction between fish oil and n-3 PUFA-enriched free fatty acid) without solvent. A bioreactor system was constructed composed of a water-jacketed packed-bed column and a substrate reservoir with a circulation pipeline between the packed-bed column and the reservoir. By keeping the temperature of the reservoir at −10°C (for the first 20 h), followed by −20°C (for the subsequent 40 h) during the batch acidolysis, crystals of free fatty acid appeared, which were removed intermittently by a cotton plug packed in the tip of the outlet pipe in the reservoir. The n-3 PUFA content of the triacylglycerol fraction increased a further 10% by the reduced temperature of the reservoir.

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Author information

Authors and Affiliations

  1. Laboratory of Molecular Biotechnology, Department of Applied Biological Sciences, School of Agriculture, Nagoya University, Nagoya 464-01, Chikusa-ku, Japan

    Tsuneo Yamane & Tomomasa Suzuki

  2. Plant Information Products Projects, New Technology Foundation, Eniwa, Hokkaido, Japan

    Tamotsu Hoshino

Authors
  1. Tsuneo Yamane
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  2. Tomomasa Suzuki
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  3. Tamotsu Hoshino
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Additional information

Bioreactors for Enzymatic Reaction of Fats and Fatty Acid derivatives, Part XV.

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Yamane, T., Suzuki, T. & Hoshino, T. Increasing n-3 polyunsaturated fatty acid content of fish oil by temperature control of lipase-catalyzed acidolysis. J Am Oil Chem Soc 70, 1285–1287 (1993). https://doi.org/10.1007/BF02564243

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  • DOI: https://doi.org/10.1007/BF02564243

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