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High pressure freezing and freeze substitution improve immunolabeling of S-locus specific glycoproteins in the stigma papillae ofBrassica

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Summary

High pressure freezing and freeze substitution methods significantly improve the antigenic preservation of S-locus specific glycoproteins (SLSG). The SLSG, which are implicated in the incompatibility response, are localized over the cell wall and cytoplasm. Labeling in the cytoplasm is mainly associated with dictyosomes and rough endoplasmic reticulum. Quantitative analysis show that in cryofixed papillae the labeling was enhanced by approximately 45% over the cell wall and approximately 90% over the dictyosomes compared to chemically fixed papillae.

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Authors and Affiliations

  1. Section of Plant Biology, Cornell University, Plant Science Building, 14850, Ithaca, NY, U.S.A.

    M. K. Kandasamy, M. V. Parthasarathy & M. E. Nasrallah

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  1. M. K. Kandasamy
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  2. M. V. Parthasarathy
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  3. M. E. Nasrallah
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Kandasamy, M.K., Parthasarathy, M.V. & Nasrallah, M.E. High pressure freezing and freeze substitution improve immunolabeling of S-locus specific glycoproteins in the stigma papillae ofBrassica . Protoplasma 162, 187–191 (1991). https://doi.org/10.1007/BF02562562

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  • DOI: https://doi.org/10.1007/BF02562562

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