Summary
Although methods are well established for the rapid histological preparation of fresh frozen soft tissues, they remain inadequate for the preservation of hard tissues. A recent technique for sectioning fresh frozen bone, in which sections can be prepared within an hour, has been applied to undecalcified human iliac crest bone biopsies. Quantitative analysis has shown that the static remodeling variables deduced from frozen sections closely resemble those derived from plastic-embedded sections prepared for routine use by established laboratory procedures. It is concluded that the rapid method is reliable for immediate diagnostic and histomorphometric purposes.
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References
Pearse AD, Gardner DL (1972) Preparation of unfixed undemineralised bone sections: the Bright bone cryostat. J Clin Pathol 25:26–29
Johnstone JJA (1979) The routine sectioning of undecalcified bone for cytochemical studies. Histochem J 11:359–365
Stevens A, Palmer J (1985) Frozen sections of bone biopsies in metabolic and other bone diseases. Histopathology 3:315–324
Phillpotts JC, Smith JG (1988) Treatment of bone marrow trephine biopsies prior to cryotomy for immunocytochemical demonstration of cell surface antigens. Med Lab Sciences 45:52–56
Aaron JE, Carter DH (1987) Rapid preparation of fresh frozen undecalcified bone for histological and histochemical analysis. J Histochem Cytochem 35:361–369
Carter DH (1986) The cryomicrotomy of undecalcified bone: a rapid method for morphological and histochemical analysis. M Ph Thesis, University of Leeds
Merz WA, Schenk RK (1970) Quantitative structural analysis of human cancellous bone. Acta Anat 75:54–66
Aaron JE (1978) Histological aspects of the relationship between vitamin D and bone. In: Lawson DEM (ed) Vitamin D. Academic Press, London, pp 201–265
Schenk RK (1965) Zur histologischen Verarbeitung von unentkalkten Knochen. Acta Anat 60:3–19
Aaron JE (1976) Diagnostic procedures. In: Nordin BEC (ed) Calcium, phosphate and magnesium metabolism. Churchill Livingstone, Edinburgh, pp 516–567
Schenk RK, Merz WA, Muller J (1969) A quantitative histological study on bone resorption in human cancellous bone. Acta Anat 74:44–53
Melvin KEW, Jepner GW, Bordier P, Neale G, Joplin GF (1970) Calcium metabolism and bone pathology in adult coeliac disease. Quart J Med 39:83–113
Thomas WC (1961) Comparative studies on bone matrix and osteoid by histochemical techniques. J Bone Joint Surg 43a:419–427
Aaron JE, Makins NB, Francis RM, Peacock M (1984) Staining of the calcification front in human bone using contrasting fluorochromes in vitro. J Histochem Cytochem 32:1251–1261
Meunier P, Edouard C (1973) Quantification of osteoid tissue in trabecular bone. Methodology and results in normal iliac bone. In: Jaworski ZFG (ed) Bone morphometry. University of Ottowa Press: Ottowa pp. 191–196
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Carter, D.H., Barnes, J.M. & Aaron, J.E. Histomorphometry of fresh frozen iliac crest bone biopsies. Calcif Tissue Int 44, 387–392 (1989). https://doi.org/10.1007/BF02555966
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DOI: https://doi.org/10.1007/BF02555966