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Platelet-derived growth factor enhances demineralized bone matrix-induced cartilage and bone formation

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Summary

Subcutaneous implantation of demineralized bone matrix induces the local formation of cartilage and bone. In this study we have investigated the influence of adding various growth factors to the implant. Cartilage formation was monitored by measuring collagen II mRNA levels, and bone formation in the implant was assessed from alkaline phosphatase activity and calcium content. Supplements of the platelet-derived growth factor to implants in older rats increased and production of mRNA for collagen II, alkaline phosphatase activity, and the calcium content of the implant, whereas the other growth factors tested were without effect. The data suggest that under some conditions bone induction is submaximal and can be increased by local supplement of platelet-derived growth factor (PDGF). The present observations may have important therapeutic implications in the treatment of nonunions of fractures and impaired bone formation in the aged.

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Author notes

  1. R. Howes

    Present address: Department of Anatomical Sciences, University of Oklahoma Health Science Center, Oklahoma

  2. J. M. Bowness

    Present address: Department of Biochemistry, University of Manitoba, Winnipeg, Canada

  3. G. R. Grotendorst

    Present address: Department of Medicine, University of South Carolina, Charleston, South Carolina

Authors and Affiliations

  1. National Institute of Dental Research, National Institutes of Health, Bldg. 30, 20892, Bethesda, Maryland, USA

    R. Howes, J. M. Bowness, G. R. Grotendorst, G. R. Martin & A. H. Reddi

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  1. R. Howes
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  2. J. M. Bowness
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  3. G. R. Grotendorst
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  4. G. R. Martin
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  5. A. H. Reddi
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Howes, R., Bowness, J.M., Grotendorst, G.R. et al. Platelet-derived growth factor enhances demineralized bone matrix-induced cartilage and bone formation. Calcif Tissue Int 42, 34–38 (1988). https://doi.org/10.1007/BF02555836

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  • DOI: https://doi.org/10.1007/BF02555836

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