Skip to main content
SpringerLink
Log in

Simultaneous measurement of bone formation and resorptionin vivo

  • Rapid Communications
  • Published:
Calcified Tissue International Aims and scope Submit manuscript

Summary

Bone resorption and formation in terms of milligram of calcium deposited into and released from whole humerus of 15–23 weeks old male rats were assessed, via linear least-squares regression analysis of change in calcium content and3H-tetracycline kinetics of the bone. The rate of resorption was 0.376 mg calcium per day, while formation was 0.758 mg calcium per day. The data indicate that the computation results in a simultaneous and explicit measurement of bone resorption and formation at the organ level.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Klein L, Jackman KV (1976) Assay of bone resorption in vivo with3H-tetracycline. Calcif Tiss Res 20:275–290

    Article  CAS  Google Scholar 

  2. Klein L, Wong KM, Simmelink JW (1985) Biochemical and autoradiographic evaluation of bone turnover in prelabeled dogs and rabbits on normal and calcium-deficient diets. Bone 6:395–399

    Article  PubMed  CAS  Google Scholar 

  3. Li XQ, Donovan CA, Klein, L (1989) A pharmacokinetic model in the rat and rabbit of the direct measurement of matura bone resorption IN VIVO with3H-tetracycline. J Pharma Sci In press.

  4. Edwards AL (1976) An introduction to linear regression and correlation. WH Freeman and Company, San Francisco, pp 81–113

    Google Scholar 

  5. Urist MR, Ibsen KH (1963) Chemical reactivity of mineralized tissue with oxytetracycline. Arch Path 76: 484–496

    PubMed  CAS  Google Scholar 

  6. Frost HM (1965) Tetracycline and fetal bone. Henry Ford Hosp Med Bull 13:403–410

    CAS  Google Scholar 

  7. Klein L, Wong KM (1985) Rapid effect of calcium deficiency upon bone resorption in rats prelabeled with3H-tetracycline and45Ca. Clin Res 33:889A

    Google Scholar 

  8. Klein L, Heiple KG, Stromberg BV (1983) Comparison of growth-induced resorption and denervation-induced resorption on the release of3H-tetracycline,45Calcium, and3H-collagen from whole bones of growing rats. J Orthop Res 1:50–56

    Article  PubMed  CAS  Google Scholar 

  9. Klein L, Heiple KG, Torzilli PA, Goldberg VM, Burstein AH (1989) Prevention of ligament and meniscus atrophy by active joint motion in a non-weigh-bearing model. J Orthop Res 7:80–85

    Article  PubMed  CAS  Google Scholar 

  10. Kelly RG, Buyske DA (1960) Metabolism of tetracycline in the rat and the dog. J Exp Pharmacol Therap 130:144–149

    CAS  Google Scholar 

  11. Chen TL, Klein L (1978) Fetal rat bone in organ culture: effect of bone growth and bone atrophy on the comparative losses of45Ca and3H-tetracycline. Calcif Tiss Res 25: 255–263

    Article  CAS  Google Scholar 

  12. Frost HM (1969) Tetracycline-based histological analysis of bone remodeling. Calcif Tiss Res 3:211–237

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Orthopaedic Research Laboratory, Wearn Building, University Hospitals of Cleveland, 2074 Abington Road, 44106, Cleveland, Ohio, USA

    Xiao Qing Li & LeRoy Klein

Authors
  1. Xiao Qing Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. LeRoy Klein
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, X.Q., Klein, L. Simultaneous measurement of bone formation and resorptionin vivo . Calcif Tissue Int 46, 282–283 (1990). https://doi.org/10.1007/BF02555009

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02555009

Key words

Search

Navigation