Summary
Ectopic bone formation by subcutaneously implanted demineralized bone matrix powder (DBM) was assessed biochemically and histologically in Fischer 344 rats of different ages. The total calcium accumulated in implants was greatly depressed in older rats, as was the rate of45Ca deposition. High alkaline phosphatase activity appeared later in the 10- and 16-month-old rats compared with 1-month-old rats, and the magnitude of the alkaline phosphatase activity was decreased in 16-month-old rats. The accumulation of the bone-specific vitamin K-dependent bone protein (bone gla protein, BGP) was decreased in the implants in older rats. Histological examination of the implants confirms the decreased ability of aged animals to produce bone in response to DBM. Measurements of total calcium, alkaline phosphatase, and BGP at the site of demineralized bone matrix implants clearly demonstrates that bone formation decreases dramatically with increasing age. Significant differences in total calcium can be detected even between 1-month-old and 3-month-old rats. Serum BGP shows a marked decrease (47%) between 1-month- and 3-month-old rats, a decrease not paralleled by a similar decrease in BGP present in calvarial or tibial bone.
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References
Nordin BEC, Aaron J, Speed R, Crilly RC (1981) Bone formation and resorption as the determinants of trabecular bone volume in postmenopausal osteoporosis. Lancet 11:277–279
Merz WA, Schenk RK (1970) A quantitative histological study on bone formation in human cancellous bone. Acta Anat 76:1–15
Lips P, Courpron P, Meunier PJ (1978) Mean wall thickness of trabecular bone packets in the human ileac crest: changes with age. Calcif Tissue Res 26:13–17
Urist MR (1965) Bone: formation by autoinduction. Science 150:893–899
Reddi AH, Huggins CB (1972) Biochemical sequences in the transformation of normal fibroblasts in adolescent rats. Proc Natl Acad Sci (USA) 69:1601–1605
Reddi AH (1981) Cell biology and biochemistry of endochondral bone development. Coll Res 1:209–226
Syftestad GT, Urist MR (1982) Bone aging. Clin Orthop Rel Res 162:288–297
Irving JT, LeBolt BA, Schneider EL (1981) Ectopic bone formation and aging. Clin Orthop Rel Res 154:249–253
Reddi AH, Sullivan NS (1980) Matrix-induced endochondral bone differentiation: influence of hypophysectomy, growth hormone, and thyroid-stimulating hormone. Endocrinology 107:1291–1299
Nishimoto SK, Price PA (1979) Proof that the γ-carboxyglutamic acid-containing bone protein is synthesized in calf bone. J Biol Chem 254:437–441
Price PA, Lothringer JW, Baukol SA Reddi AH (1981) Developmental appearance of the vitamin K-dependent protein of bone during calcification: analysis of mineralizing tissues in human, calf and rat. J Biol Chem 256:3781–3784
Price PA, Nishimoto SK (1980) Radioimmunoassay for the Vitamin K-dependent protein of bone and its discovery in plasma. Proc Natl Acad Sci 77:2234–2238
Price PA, Parthemore JG, Deftos LJ (1980) New biochemical marker for bone metabolism. J Clin Invest 66:878–883
Delmas PD, Wahner HW, Mann KG, Riggs BL (1983) Assessment of bone turnover in postmenopausal osteoporosis by measurement of serum bone gla-protein. J Lab Clin Med 102:470–476
Delmas PD, Stenner D, Wahner HW, Mann KG, Riggs BL (1983) Increase in serum bone γ-carboxyglutamic acid protein with aging in women. J Clin Invest 71:1316–1321
Macek K, Deyl A, Adam M (1980) The effect of age upon the content of γ-carboxyglutamic acid in rat mineralized tissue. Exp Gerontol 15:1–5
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Nishimoto, S.K., Chang, CH., Gendler, E. et al. The effect of aging on bone formation in rats: Biochemical and histological evidence for decreased bone formation capacity. Calcif Tissue Int 37, 617–624 (1985). https://doi.org/10.1007/BF02554919
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DOI: https://doi.org/10.1007/BF02554919