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Mineral induction by immobilized polyanionic proteins

  • Molecular and Cellular Biology
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Summary

The purpose of this study was to investigate the mineral induction capacityin vitro of polyanionic proteins covalently bound to a surface. Rat dentin γ-carboxyglutamate-containing protein of the osteocalcin type (Gla-protein), proteoglycan (PG), and phosphoprotein (PP-H), as well as phosvitin (PhV) and bovine serum albumin (BSA), were covalently linked to agarose beads. These were incubated at 37°C in solutions with a Ca/P molar ratio of 1.67, [Ca][P] molar products in the range 1.0–1.8 mM2, and an ionic strength of 0.165. The incubations were performed at constant pH and composition conditions; no spontaneous precipitation occurred under these conditions. Mineral formation, as monitored by scanning electron microscopy (SEM), was induced by all immobilized polyanions, including enzymatically dephosphorylated PP-H and PhV. No mineral was induced by BSA. The mineral inductive capacity of immobilized polyanionic proteins, as judged by the SEM after identical incubations, was found to differ between the different ligands. The mineral induced by PP-H and PG was shown by X-ray diffraction to be apatitic. It was concluded that, although polyanionic proteins in solution may inhibit mineral induction and growth, very minute quantities of such molecules, when immobilized on a surface, induce mineral at physiological concentrations of calcium and phosphate ions. The data presented may be taken to suggest that PP-H and PG, and perhaps other polyanions, may possibly be responsible for mineral nucleation in dentin and bone. The results, however, also point to the rather limited specificity in this type of reaction.

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Author notes

  1. Anders Linde

    Present address: Department of Oral Biochemistry, Faculty of Odontology, Gothenburg University, S-400 33, Gothenburg, Sweden

  2. Adrian Lussi

    Present address: Department of Operative, Preventive, and Pediatric Dentistry, School of Dentistry, University of Bern, 3010, Bern, Switzerland

Authors and Affiliations

  1. Dental Research Center and Departments of Endodontics and Pediatric Dentistry, University of North Carolina, Chapel Hill, North Carolina, USA

    Anders Linde, Adrian Lussi & Miles A. Crenshaw

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  2. Adrian Lussi
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  3. Miles A. Crenshaw
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Linde, A., Lussi, A. & Crenshaw, M.A. Mineral induction by immobilized polyanionic proteins. Calcif Tissue Int 44, 286–295 (1989). https://doi.org/10.1007/BF02553763

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