Summary
We present the first attempts to find molecular markers (RAPDs) in the apple genome linked to theVf gene conferring resistance to scab. The availability of genetic markers will allow selection of individuals with resistance genes in progeny. Moreover genetic markers allow us to recognise individuals with minimal linkage drag in back-crossing experiments with wild species. The feasibility of the bulk-segregant analysis to identify linked markers has been demonstrated on several occasions (Martin et al., 1991; Giovannoni et al., 1991; Michelmore et al., 1991). In this paper we report the preliminary results proving that the method could also be applied successfully in allogamous plants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alston, EH., 1989. Breeding pome fruits with stable resistance to diseases. In: C. Gessler, D. Butt & B. Koller (Eds) Integrated control of pome fruit diseases 11, pp. 90–99. OILB-WPRS Bulletin XII/6.
Chevalier, M., Y. Lespinasse & S. Renaudin, 1991. A microscopic study of the different classes of symptoms coded by the Vf gene in apple for resistance to scab (Venturia inaequalis). Plant Pathology 40:249–256.
Crosby, J.A., J. Janick, E.C. Pecknold, S.S. Korban, P.A. O'Connor, S.M. Ries, J. Goffreda & A. Voordeckers, 1992. Breeding Apples for Scab resistance: 1945–1990. Fruit Varieties Journal 46: 145–166.
Gessler, C., 1992. Scab resistance in apple: the minor genes in the Vf-resistance. Acta Phytopathol. Entomol. Hungaricae 27: 257–263.
Gessler, C., 1994. Biology and biotechnology in strategies to control apple scab. Norwegian Journal of Agricultural Sciences. In press.
Gessler, C. & Ph. Blaise, 1994. Differential resistance in apple against scab and its use in breeding and in orchard planting strategies to control the disease. In: H. Schmidt and M. Kellerhals (Eds.) Progress in temperate fruit breeding, pp. 99–104. Kluwer Academic Publishers, Dordrecht.
Giovannoni, J.J., R.A. Wing, M.W. Ganal & S.D. Tanksley, 1991. Isolation of molecular markers from specific chromosomal intervals using DNA pools from existing mapping population. Nucleic Acid Research 19: 6553–6558.
Haley, S.D., P.N. Miklas, J.R. Stavely, J. Byrum & J.D. Kelly, 1993. Identification of RAPD markers linked to a major rust resistance gene block in common bean. Theoretical and Applied Genetics 86:505–512.
Kellerhals, M., 1989a. Breeding of pome fruits with stable resistance to diseases. In: C. Gessler, D. Butt & B. Koller (Eds) Integrated control of some fruit diseases 11, pp. 116–129. OILB-WPRS Bulletin XIU6.
Kellerhals, M., 1989b. Breeding disease resistant apple cultivars in Switzerland. In: C. Gessler, D. Butt & B. Koller (Eds) Integrated control of some fruit diseases II, pp. 130–136. OILB-WPRS Bulletin XIU6.
Lespinasse, Y., 1989. Breeding of some fruits with stable resistance to diseases: Genes, resistance mechanisms, present work and prospects. In: C. Gessler, D. Butt & B. Koller (Eds) Integrated control of some fruit diseases II, pp. 100-115. OILB-WPRS Bulletin XIU6.
Martin, G.B., J.G.K. Williams & S.D. Tanksley, 1991. Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primer and near-isogenic lines. Proc. Natl. Acad. Sci. USA 88: 2336–2340.
Michelmore, R.W., I. Paran & R.V. Kesseli, 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in a specific genomic region by using segregating population. Proc. Natl. Acad. Sci. USA 88: 9828–9832.
Newbury, H.J., 1992. Fungal resistance: The isolation of a plant R gene by transposon tagging. In: Gatehouse, Hilder & Boulter (Eds) Plant Genetic Manipulation for Crop Protection, pp: 109–134, CAB. Biotechnology in agriculture No. 7: 266.
Parisi, L., Y. Lespinasse, J. Guillaumes & J. Krüger, 1993. A new race ofVenturia inaequalis virulent to apples with resistance due to theVf gene. Phytopathology 83: 533–537.
Vanderplank,J.E., 1982. Host-Pathogen Interaction in Plant Disease. Academic Press, New York, 207 pp.
Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research. 18:6531–5435.
Wolfe, M.S. & C. Gessler, 1992. Resistance genes in breeding: epidemiology: In: T. Boller & E. Meins (Eds) Plant gene research. Genes Involved in Plant Defense, pp.3–23, Springer Verlag.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gianfranceschi, L., McDermott, J.M., Seglias, N. et al. 1994 Kluwer Academic Publishers. Towards a marker assisted breeding for resistance against apple scab. Euphytica 77, 93–96 (1994). https://doi.org/10.1007/BF02551468
Issue Date:
DOI: https://doi.org/10.1007/BF02551468