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Transesterification of cocoa butter by fungal lipases: Effect of solvent on 1,3-specificity

  • Published:
Journal of the American Oil Chemists’ Society

Abstract

Transesterification and alcoholysis reactions catalyzed by immobilized lipases fromMucor miehei andHumicola lanuginosa in hexane gave fatty acid esters that did not reflect the expected 1,3-specificity of the enzymes, due to competing acyl migrations in the partial glyceride products. However, both lipases were 1,3-specific in reactions when diethyl ether was used as a solvent, and this provided a convenient analytical methodology in combination with gas chromatography and nuclear magnetic resonance spectroscopy for the determination of fatty acid distribution within triglycerides.

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Authors and Affiliations

  1. Department of Chemistry, University of Malaya, 59100, Kuala Lumpur, Malaysia

    S. H. Goh & S. K. Yeong

  2. Department of Biochemistry, University of Malaya, 59100, Kuala Lumpur, Malaysia

    C. W. Wang

Authors
  1. S. H. Goh
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  2. S. K. Yeong
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  3. C. W. Wang
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Goh, S.H., Yeong, S.K. & Wang, C.W. Transesterification of cocoa butter by fungal lipases: Effect of solvent on 1,3-specificity. J Am Oil Chem Soc 70, 567–570 (1993). https://doi.org/10.1007/BF02545320

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  • DOI: https://doi.org/10.1007/BF02545320

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