Abstract
Alterations of microsomal membrane integrity were examined during lipid peroxidation. Loss of membrane-bound NADPH cytochromec reductase activity and protein release into the aqueous phase were related to disruption of the lipophilic region of the membrane. Formation of fluorescent products in the lipid phase of the membrane occurred only in the presence of peroxidation products. Changes in the membrane lipid phase during peroxidation included a decrease in the reactive amino groups of the phospholipids and a decrease in detectable phosphatidyl ethanolamine. In an ultrafiltration continuous flow chamber, peroxidized microsomal membranes were hydrolyzed to a lesser degree by solubilized lysosomal cathepsins and to a greater degree by lysosomal nucleases than were nonperoxidized membranes.
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References
Fleischer, S., and G. Rouser, JAOCS 42:588 (1956).
Tappel, A.L., in “Pathological Aspects of Cell Membranes,” Vol. 1, Edited by B.F. Trump and A. Arstila, Academic Press, New York, In press.
Roubal, W.T., and A.L. Tappel, Arch. Biochem. Biophys. 113:150 (1966).
Roubal, W.T., Lipids 6:62 (1971).
Menzel, D.B., Ibid. 2:83 (1967).
Chio, K.S., and A.L. Tappel, Biochemistry 8:2827 (1969).
Wills, E.D., Biochem. J. 123:983 (1971).
Corliss, G.G., and L.R. Dugan, Jr., Lipids 5:846 (1970).
Rao, G.V., and L.R. Dugan, Jr., JAOCS 47:289 (1970).
May, H.E., and P.B. McCay, J. Biol. Chem. 243:2288 (1968).
Victoria, E.J., and A.A. Barber, Lipids 4:582 (1969).
Branton, D., and D.W. Deamer, Protoplasmatologia II/E 1:1 (1972).
Fujii, T., and T. Sato, Chem. Pharm. Bull. (Tokyo) 19:385 (1971).
Perkins, E.G., T.H. Joh and F.A. Kummerow, in “Metabolism of Lipids as Related to Atherosclerosis,” Edited by F.A. Kummerow, C. Thomas, Springfield, 1965, p. 48.
Harman, D., J. Amer. Geriat. Soc. 17:721 (1969).
Tappel, A.L., Geriatrics 23:97 (1968).
Zeman, W., Advan. Gerontol. Res. 3:147 (1971).
DiLuzio, N.R., and A.D. Hartman, Exp. Mol. Pathol. 11:38 (1969).
Recknagel, R.O., Pharm. Rev. 19:145 (1967).
de Duve, C., B.C. Pressman, R. Gianetto, R. Wattiaux and F. Appelmans, Biochem. J. 60:604 (1955).
Kwon, T.W., and B.M. Watts, J. Food Sci. 28:627 (1963).
Rouser, G., S. Fleischer and A. Yamamoto, Lipids 5:494 (1970).
Miller, G.L., Anal. Chem. 31:964 (1958).
Horecker, B.L., in “Methods in Enzymology,” Vol. II, Edited by S.P. Colowick and N.O. Kaplan, Academic Press, New York, 1955, p. 704.
Dallner, G., Acta Pathol. Microbiol. Scand. Suppl. 166:1 (1963).
Fiske, C.H., and Y. SubbaRow, in “Methods in Enzymology,” Vol. III, Edited by S.P. Colowick and N.O. Kaplan, Academic Press, New York, 1957, p. 843.
Metrione, R.M., A.G. Neves and J.S. Fruton, Biochemistry. 5:1597 (1966).
Ansorge, S., P. Bohley, H. Kirschke, J. Langner, and H. Hanson, Acta Biol. Med. Ger. 26:953 (1971).
Futai, M., S. Mirjata, and D. Mizuno, J. Biol. Chem. 244:4951 (1969).
Litwack, G., in “Experimental Biochemistry,” John Wiley and Sons, 1966, p. 21.
Hochstein, P., and L. Ernster, in “CIBA Symposium on Cellular Injury,” Edited by A.V.S. de Reuck and J. Knight, Little and Brown, Boston, 1964, p. 123.
Robinson, J.D., Arch. Biochem. Biophys. 112:170 (1965).
Dillard, C.J., and A.L. Tappel, Lipids 6:715 (1971).
Bidlack, W.R., Ph.D. thesis, University of California, Davis, 1972.
Tappel, A.L., Ann. N.Y. Acad. Sci. 203:12 (1972).
Gruger, E.H., and A.L. Tappel, Lipids 6:147 (1971).
Tinberg, H.M., and A.A. Barber, J. Nutr. 100:413 (1970).
Porta, E.A., and W.S. Hartroft, in “Pigments in Pathology,” Edited by M. Wolman, Academic Press, New York, 1969, p. 191.
Chio, K.S., and A.L. Tappel, Biochemistry 8:2821 (1969).
Arstila, A.U., M.A. Smith, and B.F. Trump, Science 175:530 (1972).
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Bidlack, W.R., Tappel, A.L. Damage to microsomal membrane by lipid peroxidation. Lipids 8, 177–182 (1973). https://doi.org/10.1007/BF02544631
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DOI: https://doi.org/10.1007/BF02544631