Skip to main content
SpringerLink
Log in

Embryonic fatty acid composition as a function of yolk fatty acid composition in eggs of the lesser spotted dogfish (Scyliorhinus canicula L.)

  • Published:
Lipids

Abstract

Yolk and embryonic total lipids were extracted from spotted dogfish eggs at two developmental stages. Total lipids were fractionated into neutral lipids (NL) and polar lipids (PL), and the fatty acid composition of each group was determined. Yolk lipid composition was found to be quantitatively different (NL/PL≊1) from embryo lipid composition (NL/PL≊0.5), for both stages of development. However, individual fatty acid composition did not differ from younger to older eggs for either yolk or embryo. There were significant differences (p<0.05) in major fatty acid groups from yolk and embryonic PL for saturated fatty acids, monounsaturated fatty acids (MUFA) and n−3 polyunsaturated fatty acids (PUFA) for younger eggs, and for MUFA and n−3 PUFA for older eggs. For NL, only MUFA composition from the oldest eggs showed differences between yolk and embryo. Results are discussed in terms of embryonic needs for highly unsaturated fatty acid (HUFA) biosynthesis, as well as to provide some explanations for the unusually high levels of 20∶4n−6 in both yolk and embryonic neutral lipids and polar lipids.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

FAME:

fatty acid methyl esters

HUFA:

highly unsaturated fatty acids

MUFA:

monounsaturated fatty acids

NL:

neutral lipids

PL:

polyunsaturated acids

PUFA:

polyunsaturated fatty acids

TLC:

thin-layer chromatography

References

  1. Kanazawa, A., Teshiwa, S.I., and Ono, K. (1979)Comp. Biochem. Physiol. 63B, 295–298.

    CAS  Google Scholar 

  2. Greene, D.H.S., and Selivonchick, P. (1987)Prog. Lipid Res. 26, 53–85.

    Article  PubMed  CAS  Google Scholar 

  3. Bell, M.V., Henderson, R.J., and Sargent, J.R. (1986)Comp. Biochem. Physiol. 83B, 711–719.

    CAS  Google Scholar 

  4. Mourente, G., and Odriozola, J.M. (1990)Fish Physiol. Biochem. 8, 103–110.

    Article  CAS  Google Scholar 

  5. Diez, J.M., and Davenport, J. (1987)J. mar. biol. Ass. U.K. 67, 249–261.

    Article  Google Scholar 

  6. Dodd, M.H.I., and Duggan, R.T. (1982)Gen. Comp. Endocrinol. 46, 392–396.

    Google Scholar 

  7. Diez, J.M., and Davenport, (1990)J. Comp. Biochem. Physiol., 96B, 825–830.

    Article  CAS  Google Scholar 

  8. Mellinger, L., Wrisez, F., Alluchon-Gerard, M.J., and Goussot, C. (1985)Ichtyophysiol. Acta 9, 243–262.

    Google Scholar 

  9. Folch, J., Lees, M., and Sloane-Stanley, G.H. (1957)J. Biol. Chem. 226, 497–509.

    PubMed  CAS  Google Scholar 

  10. Holland, D., Crisp, D.J., and East, J. (1983)Mar Biol. Lett. 4, 259–265.

    CAS  Google Scholar 

  11. Morrison, V.R., and Smith, L.M. (1964)J. Lipid Res. 5, 600–608.

    PubMed  CAS  Google Scholar 

  12. Bailey, N.T. (1985)Statistical Methods in Biology, 2nd ed., Hodder and Stoughton, London.

    Google Scholar 

  13. Tocher, D.R., and Sargent, J.R. (1984)Lipids 19, 492–499.

    Article  CAS  Google Scholar 

  14. Falk-Petersen, S., Falk-Petersen, I., Sargent, J.R., and Haug, T. (1986)Aquaculture 52, 207–211.

    Article  CAS  Google Scholar 

  15. Ulvund, K.A., and Grahl-Nielsen, O. (1988)Can. J. Fish. Aquat. Sci. 45, 898–901.

    Article  CAS  Google Scholar 

  16. Lyle, J.M. (1983)J. Fish Biol. 23, 725–737.

    Article  Google Scholar 

  17. Paradis, M., and Ackman, R.G. (1977)Lipids 12, 170–176.

    Article  PubMed  CAS  Google Scholar 

  18. Exter, J., and Weihrauch, J.L. (1976)J. Amer. Dietet. Assoc. 69, 243–248.

    Google Scholar 

  19. Chacko, G.K., Goldman, D.E., and Pennock, B.E. (1972)Biochim. Biophys. Acta 280, 1–16.

    PubMed  CAS  Google Scholar 

  20. Bell, M.V., Simpson, C.M.F., and Sargent, J.R. (1983)Lipids 18, 720–726.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Bjoquimica y Biologia Molecular y Fisiologia, Facultad de Ciencias, Universidad de Valladolid, 47005, Valladolid, Spain

    Jose Manuel Diez

  2. Marine Biology Laboratories, School of Ocean Sciences, Menai Bridge, LL59 5EY, Gwynedd, U.K.

    John Davenport

Authors
  1. Jose Manuel Diez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John Davenport
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Diez, J.M., Davenport, J. Embryonic fatty acid composition as a function of yolk fatty acid composition in eggs of the lesser spotted dogfish (Scyliorhinus canicula L.). Lipids 25, 724–728 (1990). https://doi.org/10.1007/BF02544041

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02544041

Keywords

Search

Navigation