Skip to main content
SpringerLink
Log in

The raw material, finished products and dust pad analysis of detergent proteases using a small synthetic substrate

  • Technical
  • Surfactants & Detergents Technical
  • Published:
Journal of the American Oil Chemists Society

Abstract

A new method is presented that utilizes p-nitroanilide substrates to quantify proteolytic activity of detergent enzymes. The method is contrasted to the dimethylcasein method currently in use by detergent industries, and the advantages of the new method and its potential for monitoring enzyme dust in factories are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Newhouse, M.L., B. Tagg, S.J. Pocock and A.C. McEwan,Lancet i:689 (1970).

    Article  Google Scholar 

  2. Flindt, M.L., Ibid.:1177 (1969).

    Article  Google Scholar 

  3. Pepys, J., F.E. Hargreaves, J.L. Longbottom and J. Faux, Ibid.:1181 (1969).

    Article  Google Scholar 

  4. American Conference of Governmental Industrial Hygienists (ACGIH), Documentation of Threshold Limit Values, 4th edn., Cincinnati, Ohio, ACGIH, 1980.

    Google Scholar 

  5. Bruce, C.F., E. Dunn, R. Brotherton, D.R. Davies, F. Hall and S.C.M. Potts,Ann. Occup. Hyg. 21: (1978).

  6. Dunn, E., and R. Brotherton,Analyst 96:159 (1971).

    Article  CAS  Google Scholar 

  7. Brash, J.L., and D.J. Lyman, inThe Chemistry of Biosurfaces, edited by M.L. Hair, Vol. I, Marcel Dekker, New York, N.Y., 1971, p. 177.

    Google Scholar 

  8. DeMar, E.G., C. Largmen, J.W. Brodrick and M. GeokasAnalyt. Biochem. 99:316 (1979).

    Article  Google Scholar 

  9. Anson, M.L.,J. General Physiol. 22:89 (1938).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Procter & Gamble Company, Ivorydale Technical Center, 5299 Spring Grove Ave, 45217, Cincinnati, Ohio

    T. M. Rothgeb, B. D. Goodlander, P. H. Garrison & L. A. Smith

Authors
  1. T. M. Rothgeb
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. D. Goodlander
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. H. Garrison
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. A. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Rothgeb, T.M., Goodlander, B.D., Garrison, P.H. et al. The raw material, finished products and dust pad analysis of detergent proteases using a small synthetic substrate. J Am Oil Chem Soc 65, 806–810 (1988). https://doi.org/10.1007/BF02542537

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02542537

Keywords

Search

Navigation