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The isolation of omega-3 polyunsaturated fatty acids and methyl esters of fish oils by silver resin chromatography

  • Technical
  • Published:
Journal of the American Oil Chemists’ Society

Abstract

Multigram quantities of the highly unsaturated ω3 component from samples of fish oil fatty acids and esters were isolated by silver resin chromatography. An XN1010 resin column saturated with silver ions was utilized. Polyunsaturated fatty acid (PUFA) esters from fish oil concentrate (FOC) were fractionated based on the number of double bonds by using solvent programming (acetonitrile in methanol). Larger samples (4–9 g) of FOC acids and esters and menhaden acids and esters were enriched in ω3 polyunsaturates to 82–99% (95–99% total PUFA) by use of a large 100% silver resin column and isocratic elution with 30, 35 or 45% acetonitrile in acetone.

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Authors and Affiliations

  1. Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, IL

    R. O. Adlof & E. A. Emken

Authors
  1. R. O. Adlof
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  2. E. A. Emken
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Adlof, R.O., Emken, E.A. The isolation of omega-3 polyunsaturated fatty acids and methyl esters of fish oils by silver resin chromatography. J Am Oil Chem Soc 62, 1592–1595 (1985). https://doi.org/10.1007/BF02541694

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  • DOI: https://doi.org/10.1007/BF02541694

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