Skip to main content
SpringerLink
Log in

Chromatographic separation of glucopyranosyl sinapate from canola meal

  • Short Communications
  • Published:
Journal of the American Oil Chemists’ Society

Abstract

The compound 1-O-β-D glucopyranosyl sinapate (GPS), a phenolic glycoside, was separated from ethanolic extracts of defatted canola meal by a two-step chromatographic method. The first step involved Sephadex LH-20 chromatography with methanol as the eluting solvent. The solvent from the fraction containing GPS was evaporated, and glucopyranosyl sinapate was subsequently separated by a semi-preparative high-performance liquid chromatography method with an RP-18 column and a mobile phase consisting of water/acetonitrile/acetic acid (88∶10∶2, vol/vol/vol).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Kozlowska, H., R. Zadernowski and F.W. SosulskiNahrung 27:449 (1983).

    Article  CAS  Google Scholar 

  2. Dabrowksi, K.J., and F.W. Sosulski,J. Agric. Food Chem. 32:128 (1984).

    Article  Google Scholar 

  3. Zadernowksi, R.,Acta Acad. Agricult. Technol. Olst. Technologia Alimentorium 21F:3 (1987).

    Google Scholar 

  4. Wanasundara, U., R. Amarowicz and F. Shahidi,J. Agric Food Chem., in press (1994).

  5. Linscheid, M.,Z. Naturforsch. 35c:907 (1980).

    CAS  Google Scholar 

  6. Strack, Z.,Z Pflanzenphysiol 84:139 (1977).

    CAS  Google Scholar 

  7. Amarowicz, R., H. Kozlowska, M. Shimoyamada and K. Okubo,Pol. J. Food Nutr. Sci. 1/42:89 (1992).

    Google Scholar 

  8. Barton, G.M., R.S. Evans and J.A.F. Gardner,Nature 170:249 (1952).

    Article  CAS  Google Scholar 

  9. Naczk, M., J. Wanagundara and F. Shahidi,J. Agric. Food Chem. 40:444 (1992).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Memorial University of Newfoundland, St. John’s, A1B 3X9, NF, Canada

    Ryszard Amarowicz & Fereidoon Shahidi

  2. Department of Chemistry, Memorial University of Newfoundland, St. John’s, A1B 3X9, NF, Canada

    Fereidoon Shahidi

Authors
  1. Ryszard Amarowicz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fereidoon Shahidi
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Amarowicz, R., Shahidi, F. Chromatographic separation of glucopyranosyl sinapate from canola meal. J Am Oil Chem Soc 71, 551–552 (1994). https://doi.org/10.1007/BF02540674

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02540674

Key Words

Search

Navigation