Abstract
Five commercial silicas [Nova-Pak, μPorasil (Waters, Milford, MA), Kromasil (Eka Nobel, Bohus, Sweden) Spherisorb (Sigma, St. Louis, MO) and Select-KP (Biotage, Charlottesville, VA)] were tested for their efficiency in separating vitamin E and γ-oryzanol components from saponified rice bran extracts, with the perspective of future large-scale purification of the various compounds. The effects of mobile phase composition, eluent flow rate, column temperature and the physical properties of the silica on the selectivity and analysis time were studied. All silicas except Select-KP gave baseline separation of the vitamin E components with isooctane/ethyl acetate (97.5∶2.5) at room temperature. However, only the 4-μm Nova-Pak spherical silica gave adequate resolution of the oryzanols into two distinct fractions with an analysis time ofcirca 20 min. Other analytical conditions, i.e., the content of the polar modifier in the mobile phase, temperature and flow rate, can be used to optimize the separation once the silica had been chosen. Speed of separation is enhanced by increasing the polarity of the eluent, temperature and flow rate, while the selectivity is reduced only slightly. The Nova-Pak silica appears resistant to deactivation from both residual water in the mobile phase and polar components left over after sample clean-up and is suitable for preparative separation of the target compounds.
Similar content being viewed by others
References
Fereidoon, S., P.K. Janita and P.D. Wanasundara,Crit. Rev. Food Sci. and Nutr. 32:67 (1992).
Taylor, P. and P. Barnes,Chem. and Ind. 20:722 (1981).
Tan, B., and L. Bruzuskiewicz,Anal. Biochem. 180:368 (1989).
Komiyama, K., K. Iizuka, M. Yamaoka, H. Watanabe, N. Tsuchiya and I. Umezawa,Chem. Pharm. Bull. 37:1369 (1989).
De Leenheer, A.P., W.E. Lambert and H.J. Nelis,Modern Chromatographic Analysis of Vitamins, Marcel Dekker, New York, 1992, p. 153.
Bourgeois, C.,Determination of Vitamin E: Tocopherols and Tocotrienols, Elsevier Applied Science, London and New York, 1992.
Rogers, E.J., S.M. Rice, R.J. Nicolosi, D.R. Carpenter, C. A. McClelland and L.J. Romanczyk, Jr.,J. Am. Oil Chem. Soc. 70:301 (1993).
Seethamaraiah, G.S., T.P. Krishnakantha and N. Chandrasekhara,J. Nutr. Sc. Vit. 36:291 (1990).
Yoshino, G., T. Kazumi, M. Amano, M. Tateiwa, T. Yamasaki, S. Baba,Curr. Ther. Res. 45:975 (1989).
Nakamur, H.,Radioisotopes 25:371 (1966).
Nicolosi, R.J., E.J. Rogers, L.M. Ausmann and F.T. Orthoefer, inRice Science and Technology, Marcel Dekker, New York, 1993, pp. 421–431.
De Leenheer, A.P., W.E. Lambert and H.J. Nelis,Modern Chromatographic Analysis of Vitamins, Marcel Dekker, New York, 1992, p. 163.
Cort, W.M., T.S. Vicente, E.H. Waysek and B.D. Williams,J. Agric. Food Chem. 31:1333 (1983).
McMurray, C.H., W.J. Blanchflower and D.A. Rice,J. Assoc. Off. Anal. Chem. 63:1258 (1980).
Shin, T. and J.S. Godber,J. Am. Oil Chem. Soc. 70:1289 (1993).
Show, C.K., H.H. Draper and A.S. Csallany,Anal. Biochem. 32:81 (1969).
Widicus, W.A. and J.R. Kirk,J. Assoc. Off. Anal. Chem. 62:637 (1979).
Thompson, J.N., and G. Hatina,J. Liquid. Chromatogr. 2:327 (1979).
De Leenheer, A.P., W.E. Lambert and H.J. Nelis,Modern Chromatographic Analysis of Vitamins, Marcel Dekker, New York, 1992, p. 161.
Bradcock, N.R., D.A. O'Reilly and C. Pinnock,J. Chromatogr. 380:290 (1986).
Weber, E.J.,J. Am. Oil Chem. Soc. 64:1129 (1987).
Indyk, H.E.,Analyst 113:1217 (1988).
Rammell, C.G. and J.J.L. Hoogenboom,J. Liq. Chromatogr. 8:707 (1985).
Vatassery, G.T. and D.F. Hagen,Anal. Biochem. 79:129 (1977).
Vatassery, G.T., V.R. Maynard and D.F. Hagen,J. Chromatogr. 161:299 (1978).
Hess, J.L., M.A. Pallansch, K. Haric and G.E. Bunce,Anal. Biochem. 83:401 (1977).
Carpenter, A.P.,J. Am. Oil Chem. Soc. 56:668 (1978).
De Lumen, B.O. and S. Fiad,J. Agric. Food Chem. 30:50 (1982).
Snyder, L.R., J.L. Glajch, and J.J. Kirkland, inPractical HPLC Method Development, John Wiley and Sons, New York, 1988, p. 142.
Engelhardt, H. and H. Muller,J. Chromatogr. 218:395 (1981).
Scott, R.P. and P. Kucera,J. Chrom. Sci. 12:473 (1974).
Scott, R.W. and P. Kucera,J. Chromatogr. 171:37 (1979).
Scott, R.P.W.,J. Chrom. Sci. 18:297 (1980).
In-Hwan, K. and K. Chul-Jin,Korean J. Food Sci. 23:76 (1991).
Golshan-Shirazi, S. and G. Guiochon,J. Chromatogr. 517:57 (1990).
Golshan-Shirazi S. and G. Guiochon,Anal. Chem. 60:2364 (1988).
Golshan-Shirazi, S. and G. Guiochon,6:462 (1989).
Ha, L., and A. Saari Csallany,Lipids 23:359 (1988).
Tanaka, A, A. Kato and T. Tsuchiya,J. Am. Oil Chem. Soc. 48:95 (1971).
Author information
Authors and Affiliations
About this article
Cite this article
Diack, M., Saska, M. Separation of vitamin E and γ-oryzanols from rice bran by normal-phase chromatography. J Am Oil Chem Soc 71, 1211–1217 (1994). https://doi.org/10.1007/BF02540539
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02540539