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Separation of monoacylglycerols by high-performance liquid chromatography on nitrile-bonded phase

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Journal of the American Oil Chemists’ Society

Abstract

Monoacylglycerol molecular species, as their di-3,5-dinitrophenylurethane derivatives, were well separated by normalphase high-performance liquid chromatography on nitrilebonded phase. The peaks emerged in the order 20∶0, 18∶0, 16∶0, 18∶1, 16∶1, 18∶2, and 18∶3. The peaks of 1- and 2-monoacylglycerols with the same acyl group showed complete overlapping. This method could be applied to get acyl compositions in the three positions of triacyl-sn-glycerols in their stereospecific analysis.

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References

  1. Itabashi, Y., and T. Takagi,Lipids 21:413 (1986).

    Article  CAS  Google Scholar 

  2. Takagi, T., and Y. Ando,.25:398 (1990).

    Article  CAS  Google Scholar 

  3. Takagi, T., and Y. Ando,.26:542 (1991).

    Article  CAS  Google Scholar 

  4. Ando, Y., K. Nishimura, N. Aoyanagi and T. Takagi,J. Am. Oil Chem. Soc., 69:417 (1992).

    CAS  Google Scholar 

  5. Christie, W.W., B. Nikolova-Damyanova, P. Laakso and B. Herslof,68:695 (1991).

    CAS  Google Scholar 

  6. Santinelli, F., P. Damanni and W.W. Christie,69:552 (1992).

    CAS  Google Scholar 

  7. Ando, Y., and T. Takagi,70:1047 (1993).

    CAS  Google Scholar 

  8. Sebedio, J.L., inCRC Handbook of Chromatography Analysis of Lipids, edited by K.D. Mukherjee, and N. Weber, CRC Press, Boca Raton, 1993, p. 57.

    Google Scholar 

  9. Yoo, J.S., and V.L. McGuffin,J. Chromatogr., 627:87 (1992).

    Article  CAS  Google Scholar 

  10. Fatemi, S.H., and E.G. Hammond,Lipids 12:1032 (1977).

    Article  CAS  Google Scholar 

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Author notes

  1. Toru Takagi

    Present address: c/o Toru Ota, Department of Chemistry, Faculty of Fisheries, Hokkaido University, Minato-cho, 041, Hakodate, Japan

Authors and Affiliations

  1. c/o Toru Ota, Department of Chemistry, Faculty of Fisheries, Hokkaido University, Minato-cho, 041, Hakodate, Japan

    Yasuhiro Ando

Authors
  1. Toru Takagi
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  2. Yasuhiro Ando
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Takagi, T., Ando, Y. Separation of monoacylglycerols by high-performance liquid chromatography on nitrile-bonded phase. J Am Oil Chem Soc 71, 459–460 (1994). https://doi.org/10.1007/BF02540532

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  • DOI: https://doi.org/10.1007/BF02540532

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