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Similarities between a UV-sensitive mutant of yeast and bacterial mutants lacking excision-repair ability

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Summary

A UV-sensitive and a wild-type strain ofSaccharomyces cerevisiae have been compared with respect to their responses to photoreactivation, retention of the capacity to photoreactivate when stored at 32°C in buffer, and sensitivity to diepoxybutane and nitrosoguanidine. In all these tests the behaviour of the sensitive mutant paralleled bacterial strains lacking excision repair ability. We may tentatively attribute the UV sensitivity in this mutant to a loss of some element of a repair system analogous to excision repair in bacteria.

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References

  • Chang, L. T., andR. W. Tuveson: U.V.-sensitive mutants inNeurospora crassa. Genetics56, 801–810 (1967).

    PubMed  CAS  Google Scholar 

  • Cox, B. S., andJ. M. Parry: The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeast. Mutation Res.6, 37–55 (1968).

    PubMed  CAS  Google Scholar 

  • Dulbecco, R.: Photoreactivation. In: Radiation biology, p. 455 et seq. vol. II (ed.A. Hollaender). New York: McGraw Hill Book Co. 1955.

    Google Scholar 

  • Haefner, K., andL. Howrey: Gene controlled U.V.-sensitivity inSchizosaccharomyces pombe. Mutation Res.4, 219–221 (1967).

    Google Scholar 

  • Harm, W.: Dark repair of photorepairable U.V. lesions inEscherichia coli. Mutation Res.6, 25–35 (1968).

    PubMed  CAS  Google Scholar 

  • —, andK. Haefner: Decreased survival resulting from liquid holding recovery of U.V. irradiatedEscherichia coli C andSchizosaccharomyces pombe. Mutation Res.8, 179–192 (1968).

    CAS  Google Scholar 

  • Hill, R. F., andE. Simpson: A study of radiosensitive and radioresistant mutants ofEscherichia coli strain B. J. gen. Microbiol.24, 1–14 (1961).

    PubMed  CAS  Google Scholar 

  • Holliday, R.: Radiation sensitive mutants ofUstilago maydis. Mutation Res.2, 557–599 (1965).

    PubMed  CAS  Google Scholar 

  • Jagger, J.: A small and inexpensive U.V. dose-rate meter useful in biological experiments. Radiat. Res.14, 394–403 (1961).

    Article  PubMed  CAS  Google Scholar 

  • Kilbey, B. J.: Diepoxybutane pretreatment effects on U.V.-inactivation and photoreactivation ofSaccharomyces cerevisiae. Mutation Res. (1969) (in press).

  • Nakai, S., andS. Matsumoto: Two types of radiation sensitive mutant in yeast. Mutation Res.4, 129–136 (1967).

    PubMed  CAS  Google Scholar 

  • Ross, W. C. J.: Biological alkylating agents. London: Butterworths 1962.

    Google Scholar 

  • Setlow, R. B., andW. L. Carrier: The disappearance of thymine dimers from DNA; an error correcting mechanism. Proc. nat. Acad. Sci. (Wash.)57, 226–231 (1964).

    Article  Google Scholar 

  • Snow, R.: Mutants of yeast sensitive to ultraviolet light. J. Bact.94, 571–575 (1967).

    PubMed  CAS  Google Scholar 

  • Witkin, E., in: Proceedings of Symposium on “Recovery repair mechanisms in radiobiology”. Brookhaven Nat. Lab. 1968.

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Authors and Affiliations

  1. Mutagenesis Unit, Department of Genetics, University of Edinburgh, 9, Edinburgh, Scotland

    B. J. Kilbey & Sheena M. Smith

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  1. B. J. Kilbey
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  2. Sheena M. Smith
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Communicated byH. Böhme

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Kilbey, B.J., Smith, S.M. Similarities between a UV-sensitive mutant of yeast and bacterial mutants lacking excision-repair ability. Molec. Gen. Genet. 104, 253–257 (1969). https://doi.org/10.1007/BF02539289

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  • DOI: https://doi.org/10.1007/BF02539289

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