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Fatty chain compositon of ether and ester glycerophospholipids in the Japanese oysterCrassostrea gigas (Thunberg)

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Lipids

Abstract

The fatty chain compositions of 1-O-alk-1′-enyl-2-acyl, 1-0-alkyl-2-acyl, and 1,2-diacyl glycerophospholipids of the Japanese oysterCrassostrea gigas (Thunberg) were investigated. Major fatty chains in thesn-1 position of 1-alk-1′-enyl-2-acyl ethanolamine phospholipids (EPL) were 18∶0 (64.7%) and 20∶1 (11.1%). Majorsn-1 chains of alkenylacyl choline phospholipids (CPL) were 18∶0 (63.3%) and 16∶0 (22.2%). In the case of 1-alkyl-2-acyl EPL, the predominant fatty chains in thesn-1 position were 18∶0 (51.5%), 16∶0 (16.0%) and 20∶1 (12.5%); in the case of 1-alkyl-2-acyl CPL, the majorsn-1 chains were 16∶0 (44.0%) and 14∶0 (23.4%). Saturated fatty chains were predominant in both EPL and CPL. Prominent fatty acids in thesn-2 position of the alkenylacyl EPL were 22∶6n−3 (29.0%), 20∶5n−3 (19.0%) and 22∶2 NMID (non-methylene interrupted dienes, 16.6%) contributing to about 65% of the total fatty acids, while alkenylacyl CPL was rich in the saturated acids 16∶0 (32.0%) and 18∶0 (9.2%). In the alkylacyl EPL, 16∶0, 18∶1n−9, 18∶0 and 16∶1n−7 were prominentsn-2 fatty acids and accounted for 30.6%, 10.0%, 9.8%, and 8.3%, respectively. Polyunsaturated fatty acids were detected, but were present at extremely low percentages. Majorsn-2 fatty acids in alkylacyl CPL were 16∶0 (25.4%), 22∶6n−3 (16.0%) and 20∶5n−3 (8.4%). The major fatty acids of diacyl EPL were 20∶5n−3 (22.3%), 16∶0 (17.9%), and 18∶0 (16.1%), and those of diacyl CPL were 16∶0 (30.4%), 20∶5n−3 (17.6%) and 18∶1n−7 (7.4%).

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Abbreviations

CI:

chemical ionization

CPL:

choline phospholipids

EPL:

ethanolamine phospholipids

GLC:

gas-liquid chromatography

CLPL:

choline lysophospholipids

ELPL:

ethanolamine lysophospholipids

MS:

mass spectrometry

NMID:

nonmethylene interrupted diene

IPL:

inositol phospholipids

SPL:

serine phospholipids

PUFA:

polyunsaturated fatty acids

TLC:

thin-layer chromatography

TMS:

trimethylsilyl

alkenylacylxxx:

1-O-alk-1′-enyl-2-acyl-

alkylacylxxx:

1-O-alkyl-2-acyl-

diacylxxx:

1,2-diacyl-

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Authors and Affiliations

  1. Department of Food Science and Technology, The Tokyo University of Fisheries, Konan 4, Minato-ku, 108, Tokyo, Japan

    Chiaki Koizumi, Bo Young Jeong & Toshiaki Ohshima

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  1. Chiaki Koizumi
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  2. Bo Young Jeong
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  3. Toshiaki Ohshima
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Koizumi, C., Jeong, B.Y. & Ohshima, T. Fatty chain compositon of ether and ester glycerophospholipids in the Japanese oysterCrassostrea gigas (Thunberg). Lipids 25, 363–370 (1990). https://doi.org/10.1007/BF02537978

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  • DOI: https://doi.org/10.1007/BF02537978

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