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Sterol metabolism in the tobacco hornworm,Manduca sexta—A review

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Lipids

Abstract

A number of intermediates involved in the dealkylation and conversion of the major C28 and C29 phytosterols to cholesterol in insects were first isolated and identified in studies with the tobacco hornworm,Manduca sexta, carried out in our laboratory. We also investigated the effects of a variety of known sterol metabolism inhibitors inManduca, particularly those affecting the Δ24-sterol reductase enzyme, and synthesized and tested a number of new inhibitors as well. In-depth studies of ecdysteroids inManduca during embryogenesis and during pupal-adult development provided new information on molting hormone content, biosynthesis, and metabolism. In addition, this insect has been utilized in the study of three specific enzyme systems of ecdysteroid metabolism, namely 20-monooxygenase, 3-epimerase, and phosphotransferase, which are critical to activation and deactivation of molting hormones in insects.

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Abbreviations

26E26P:

26-hydroxyecdysone-26-phosphate

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Authors and Affiliations

  1. Insect Neurobiology & Hormone Laboratory, USDA, ARS, PSI, Bldg. 467, BARC-East, 20705-2350, Beltsville, MD

    James A. Svoboda & Gunter F. Weirich

Authors
  1. James A. Svoboda
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  2. Gunter F. Weirich
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Svoboda, J.A., Weirich, G.F. Sterol metabolism in the tobacco hornworm,Manduca sexta—A review. Lipids 30, 263–267 (1995). https://doi.org/10.1007/BF02537831

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  • DOI: https://doi.org/10.1007/BF02537831

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