Abstract
Changes in eicosanoid production may contribute to some of the complications of the aging process such as atherosclerosis and glomerular sclerosis. Polyunsaturated fatty acids of the n-6 and n-3 series are precursors of eicosanoids. We fed diets containing safflower oil as a source of n-6 fatty acids, fish oil as a source of n-3 fatty acids or beef tallow as a source of saturated fats to three groups of normal rats from 2–18 months of age. We demonstrated incorporation of the n-3 fatty acids, 20:5n-3 and 22:6n-3 into kidney phospholipids. Feeding of the diet containing n-3 fatty acids was associated with a markedly decreased giomerular production of PGE, 6-keto-PGF1α and TXB2. It also decreased the aortic production of 6-keto-PGF1α and platelet production of TXB2. No significant effect of n-6 fatty acids on dienoic eicosanoid production was observed. There were no adverse effects on kidney function as measured by urinary protein excretion and serum creatinine levels or on renal morphology by any diet. A diet enriched in n-3 fatty acids for 18 months remains effective in decreasing dienoic eicosanoids in the aging rat.
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Abbreviations
- ANOVA:
-
analysis of variance
- GC:
-
gas chromatograph
- HBSS:
-
Hunk's balanced salt solution
- PAS:
-
periodic acid Schiff
- PG:
-
prostaglandin
- PUFA:
-
polyunsaturated fatty acid
- RIA:
-
radioimmunoassay
- SFA:
-
saturated fatty acid
References
Hirokawa, K. (1975)Mech. Ageing Devel. 4, 301–316.
Kaplan, C., Pasternack, B., Shah, H., and Gallo, G. (1975)Am. J. Pathol. 80, 227–234.
Kappel, B., and Olsen, S. (1980)Virchows Arch. A. Path. Anat. Histol. 387, 271–277.
Kasiske, B., (1987)Kidney Int. 31, 1153–1159.
McLachlan, M.S.F., Guthrie, J.C., Anderson, C.K., and Fulker, M.J. (1977)J. Pathol. 121, 65–78.
Hollenberg, E.N., Adams, D.F., Solomon, H.S. Rashid, A., Abrams, H.L., and Merrill, J.P. (1974)Circ. Res. 34, 309–316.
Edwards, K.D.G. (1981)Controversies in Nephrology III, pp. 3–19, Georgetown University/Masson Pub., New York, NY.
Johnson, M., Ramey, E., and Ramwell, P.W., (1975)Nature 253, 355–357.
Report of the American Institute of Nutrition Ad Hoc Committee on Standards for Nutritional Studies (1977)J. Nutr. 107, 1340–1348.
Folch, J., Lees, M., and Sloane-Stanley, G.H. (1957)J. Biol. Chem. 226, 497–509.
Juaneda, P., and Rocquelin, S. (1985)Lipids 20, 40–41.
Hassid, A., Konieczkowski, M., and Dunn, M.J. (1979)Proc. Natl. Acad. Sci. USA 76, 1155–1159.
Huang, Y.S., Nassar, B.A., and Horrobin, D.F. (1986)Biochim. Biophys. Acta 879, 22–27.
Nassar, B.A., Manku, M.S., Huang, Y.S., Jenkins, D.K., and Horrobin, D.F. (1987)Prost. Leuk. Med. 26, 253–263.
Croft, K.D., Beilin, L.J., Vandongen, R., and Mathews, E. (1984)Biochim. Biophys. Acta 795, 196–207.
Croft, K.D., Codde, J.P., Barden, A., Vandongen, R., and Beilin, L. (1985)Biochim. Biophys. Acta 834, 316–323.b
Codde, J.P., and Beilin, L.J. (1985)Clin. Sci. 69, 691–699.
Codde, J.P., McGowan, H.M., Vandongen, R., and Beilin, L.J. (1985)Hypertension 7, 886–892.
Barcelli, U., and Pollak, V.E. (1985)Nephron 41, 209–212.
Klahr, S., Buerkert, J., and Purkerson, M. (1983)Kidney Int. 24, 579–587.
Prickett J.D., Robinson, D.R., and Steinberg, A.D. (1983)Arth. Rheum. 26, 133–139.
Kelley, V.E., Ferretti, A., Izui, S., and Strom, T.B. (1985)J. Immunol. 134, 1914–1919.
Kher, V., Barcelli, U., Weiss, M., Gallon, L., Pajel, P., Laskarzewski, P., and Pollak, V.E. (1986)Prost. Leuk. Med. 22, 323–334.
Barcelli, U.O., Weiss, M., and Pollak, V.E. (1982)J. Lab. Clin. Med. 100, 786–797.
Heifets, M., Morrisey, J.J., Purkerson, M., Morrison, A.R., and Klahr, S. (1987)Kidney Int. 32, 335.
Izumi, Y., Weiner, T.W., Franco-Saenz, R., and Mulrow, P.J. (1986)Proc. Soc. Exp. Biol. Med. 183, 193–198.
Barcelli, U.O., Miyata, J., Ito, Y., Gallon, L., Laskarzewski, P., Weiss, M., Hitzemann, R., and Pollak, V.E. (1986)Prostaglandins 32, 211–219.
Ito, Y., Barcelli, U., Yamashita, W., Weiss, M., Thompson, B., Kashyap, M., Deddens, J., and Pollak, V.E. (1987)Life Sci. 40, 2317–2324.
Kromhout, D., Bosschieter, E.B., and Coulander, C. (1985)New Engl. J. Med. 312, 1205–1209.
Weiner, B.H., Ockene, I.S., Levine, P.H., Cuenoud, H.F., Fisher, M., Johnson, B.F., Daoud, A.S., Jarmolych, J., Hosmer, D., Johnson, M.H., Natale, A., Vaudreuil, C., and Hoogasian, J.J. (1986)New Engl. J. Med. 315, 841–846.
Chang, W-C., Murota, S-I., Nakao, J., and Orimo, H. (1980)Biochem. Biophys. Acta 620, 159–166.
Panaganamala, R.V., Hanamaiah, B., and Merola, A.J. (1981)Prost. Med. 6, 233–235.
Lennon, E.A., and Poyser, N.L. (1986)Prost. Leuk. Med. 25 1–5.
Chang, W-C., and Tai, H-H. (1984)Life Sci. 34, 1269–1280.
Chiba, S., Abe, K., Kudo, K., Omata, K., Yasujima, M., Sato, K., Seino, M., Imai, Y., Sato, M., and Yoshinaga, K. (1984)Prost. Leuk. Med. 16, 347–358.
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Barcelli, U.O., Beach, D.C. & Pollak, V.E. The influence of n-6 and n-3 fatty acids on kidney phospholipid composition and on eicosanoid production in aging rats. Lipids 23, 309–312 (1988). https://doi.org/10.1007/BF02537339
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DOI: https://doi.org/10.1007/BF02537339