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Fatty acid metabolism and cell proliferation. III. Effect of prostaglandin biosynthesis either from exogenous fatty acid or endogenous fatty acid release with hydralazine

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Lipids

Abstract

Primary cultures of smooth muscle cells were established from the medial layer of guinea pig aorta. Cells were seeded at from 40 to 80 cells per cm2 and cloned for 8 days. Media were analyzed for PGI2 (6-keto-PGF1α) using radioimmunoassay. Prostanoids were synthesized when cells were grown in media alone. Arachidonic acid stimulated prostanoid synthesis and promoted cell proliferation. Indomethacin blocked prostanoid synthesis and abolished the stimulatory effect of arachidonic acid on cell proliferation. Hydralazine stimulated fatty acid release and prostanoid synthesis in confluent cells. Hydralazine also stimulated prostanoid synthesis and promoted proliferation in growing cells. Indomethacin blocked prostanoid synthesis and abolished the stimulatory effect of hydralazine on cell proliferation.

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References

  1. Gerschenson, L.E., Mead, J.F., Harary, I., and Haggerty, Jr., D.F. (1967) Biochim. Biophys. Acta 131, 42–49.

    Article  CAS  Google Scholar 

  2. Holley, R.W., Baldwin, J.H., and Kiernan, J.A. (1974) Proc. Natl. Acad. Sci. U.S.A. 71, 3976–3978.

    Article  PubMed  CAS  Google Scholar 

  3. Kidwell, W.R., Monaco, M.E., Wicha, M.S., and Smith, G.S. (1978) Cancer Res. 38, 4091–4100.

    PubMed  CAS  Google Scholar 

  4. Wicha, M.S., Liotta, L.A., and Kidwell, W.R. (1979) Cancer Res. 39, 426–435.

    PubMed  CAS  Google Scholar 

  5. Huttner, J.J., Gwebu, E.T., Panganamala, R.V., Milo, G.E., Cornwell, D.G., Sharma, H.M., and Geer, J.C. (1977) Science 197, 289–291.

    Article  PubMed  CAS  Google Scholar 

  6. Taylor, L., and Polgar, P. (1977) FEBS Lett. 79, 69–72.

    Article  PubMed  CAS  Google Scholar 

  7. Bettger, W.J., and Ham, R.G. (1981) Prog. Lipid Res. 20, 265–268.

    Article  PubMed  CAS  Google Scholar 

  8. de Asua, L.J., Otto, A.M., Ulrich, M., Martin-Perezi, J., and Thomas, G. (1982) in Prostaglandins and Cancer (Powles, T.J., Bockman, R.S., Honn, K.J., and Ramwell, P., eds.) pp. 309–331, Alan R. Liss, Inc., New York.

    Google Scholar 

  9. Thomas, D.R., Philpott, G.W., and Jaffe, B.M. (1974) Exp. Cell Res. 84, 40–46.

    Article  PubMed  CAS  Google Scholar 

  10. Kagen, L.J., Neigel, D.T., Collins, K., and Robinson, Jr., H.J. (1977) In Vitro 13, 18–23.

    PubMed  CAS  Google Scholar 

  11. de Mello, M.C.F., Bayer, B.M., and Beaven, M.A. (1980) Biochem. Pharmacol. 29, 311–318.

    Article  PubMed  Google Scholar 

  12. Hammarström, S. (1982) in Prostaglandins and Cancer (Powles, T.J., Bockman, R.S., Honn, K.V., and Ramwell, P., eds.) pp. 297–307, Alan R. Liss, Inc., New York.

    Google Scholar 

  13. Sinzinger, H., Silberbauer, K., Winter, M., and Auerswald, W. (1979) Exp. Path. 17, 354–356.

    CAS  Google Scholar 

  14. Moncada, S. (1982) Arteriosclerosis 2, 193–207.

    PubMed  CAS  Google Scholar 

  15. Honn, K.V., Meyer, J., Neagos, G., Henderson, T., Westley, C., and Ratanatharathorn, V. (1982) in Progress in Clinical and Biological Research, Vol. 89, Interaction of Platelet and Tumor Cells (Jamieson, G.A., ed.) pp. 295–331, Alan R. Liss, Inc., New York.

    Google Scholar 

  16. Huttner, J.J., Cornwell, D.G., and Milo, G.E. (1977) T.C.A. Manual 3, 633–639.

    Article  Google Scholar 

  17. Groschel-Stewart, U., Chamley, J.H., McConnell, J.D., and Burnstock, G. (1975) Histochemistry 43, 215–224.

    Article  PubMed  CAS  Google Scholar 

  18. Gavino, V.C., Milo, G.E., and Cornwell, D.G. (1982) Cell Tissue Kinet. 15, 225–231.

    PubMed  CAS  Google Scholar 

  19. Morisaki, N., Stitts, J.M., Bartels-Tomei, L., Milo, G.E., Panganamala, R.V., and Cornwell, D.G. (1982) Artery 11, 88–107.

    PubMed  CAS  Google Scholar 

  20. Levine, L., Gutierrez Cernosek, R.M., and Van Vunakis, H. (1971) J. Biol. Chem. 246, 6782–6785.

    PubMed  CAS  Google Scholar 

  21. Gavino, V.C., Miller, J.S., Dillman, J.M., Milo, G.E., and Cornwell, D.G. (1981) J. Lipid Res. 22, 57–62.

    PubMed  CAS  Google Scholar 

  22. Morisaki, N., Sprecher, H., Milo, G.E., and Cornwell, D.G. (1982) Lipids 17, 893–899.

    PubMed  CAS  Google Scholar 

  23. Cornwell, D.G., Huttner, J.J., Milo, G.E., Panganamala, R.V., Sharma, H.M., and Geer, J.C. (1979) Lipids 14, 194–207.

    Article  PubMed  CAS  Google Scholar 

  24. Greenwald, J.E., Wong, L.K., Alexander, M., and Bianchine, J.R. (1980) in Advances in Prostaglandins and Thromboxane Research, Vol. 6, (Samuelsson, B., Ramwell, P.W., and Paoletti, R., eds.) pp. 293–295, Raven Press, New York.

    Google Scholar 

  25. Dyer, R.D., Huttner, J.J., Tan, S.Y., and Mulrow, P.J. (1982) Prog. Lipid Res. 20, 557–560.

    Article  Google Scholar 

  26. Zusman, R.M., and Keiser, H.R. (1977) J. Clin. Invest. 60, 215–223.

    Article  PubMed  CAS  Google Scholar 

  27. Rozengurt, E., Legg, A., and Petrican, P. (1979) Proc. Natl. Acad. Sci. USA 76, 1284–1287.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Physiological Chemistry, The Ohio State University, 43210, Columbus, OH

    N. Morisaki, J. A. Lindsey, G. E. Milo & D. G. Cornwell

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  1. N. Morisaki
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  2. J. A. Lindsey
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  3. G. E. Milo
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  4. D. G. Cornwell
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Morisaki, N., Lindsey, J.A., Milo, G.E. et al. Fatty acid metabolism and cell proliferation. III. Effect of prostaglandin biosynthesis either from exogenous fatty acid or endogenous fatty acid release with hydralazine. Lipids 18, 349–352 (1983). https://doi.org/10.1007/BF02537230

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  • DOI: https://doi.org/10.1007/BF02537230

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