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Fatty acid distribution in the phospholipids ofFrancisella tularensis

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Lipids

Abstract

Francisella tularensis, LVS (live vaccine strain) grown in a chemically defined medium was found to have a lipid content of 21% by dry weight. The two major phospholipids were identified as phosphatidylethanolamine (PE; 76%) and phosphatidylglycerol (PG; 24%) by thin layer chromatographic analysis, staining characteristics and quantitative chemical analyses of fatty acid, phosphate and glycerol constituents. PE contained a high proportion of 24∶0 fatty acid, with lesser amounts of 24∶1, 22∶0 and 10∶0. The major fatty acids of PG were 18∶1 and 22∶0. Hydroxy fatty acids, which are prominent components ofF. tularensis, were conspicuously lacking in these phospholipids; it is therefore concluded that hydroxy fatty acids are constituents of other structures of the organism.

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References

  1. Hood, A.M. (1977)J. Hyg. 79, 47–60.

    Article  CAS  Google Scholar 

  2. Carlsson, H.E., Lindberg, A.A., Lindberg, G., Hederstedt, B., Karlsson, K.-A., and Agell, B.O. (1979)J. Clin. Microbiol., 10, 615–621.

    PubMed  CAS  Google Scholar 

  3. Pavlova, I.B., Meshcheryakova, I.S., and Emelyanova, O.S. (1967)J. Hyg. Epidemiol. Microbiol. Immunol. 11, 320.

    PubMed  CAS  Google Scholar 

  4. Ormsbee, R.A., and Larson, C.L. (1955)J. Immunol. 74, 351–358.

    PubMed  CAS  Google Scholar 

  5. Prochazka, O., and Dubanska, H. (1972)Folia Microbiol. 17, 232–238.

    CAS  Google Scholar 

  6. Sandstrom, G., Tarnvik, A., Wolf-Watz, H., and Lofgren, S. (1984)Infect. Immun. 45, 101–106.

    PubMed  CAS  Google Scholar 

  7. Jantzen, E., Berdal, B.P., and Omland, T. (1979)J. Clin. Microbiol. 10, 928–930.

    PubMed  CAS  Google Scholar 

  8. Lambert, M.A., and Moss, C.W. (1983)J. Clin. Microbiol. 18, 1370–1377.

    PubMed  CAS  Google Scholar 

  9. Nichols, P.D., Mayberry, W.R., Antworth, C.P., and White, D.C. (1985)J. Clin. Microbiol. 21, 738–740.

    PubMed  CAS  Google Scholar 

  10. Chamberlain, R.E. (1965)Appl. Microbiol. 13, 232–235.

    PubMed  CAS  Google Scholar 

  11. Bligh, E.C., and Dyer, W.T. (1959)Can. J. Biochem. Physiol. 37, 911–917.

    PubMed  CAS  Google Scholar 

  12. Rouser, G., Fleischer, S., and Yamamoto, A. (1970)Lipid 5, 494–496.

    Article  CAS  Google Scholar 

  13. Vaskovsky, V.E., and Kostetsky, E.Y. (1968)J. Lipid Res. 9, 396.

    PubMed  CAS  Google Scholar 

  14. Marinetti, G.V. (1962)J. Lipid Res. 3, 1–20.

    CAS  Google Scholar 

  15. Chen, R.F. (1967)J. Biol. Chem. 242, 173–181.

    PubMed  CAS  Google Scholar 

  16. Renkonen, O. (1962)Biochim. Biophys. Acta 56, 367–369.

    Article  PubMed  CAS  Google Scholar 

  17. Jantzen, E., Bryn, K., and Bovre, K. (1974)Acta Path. Microbiol. Scand. Sect. B, 82, 753–766.

    CAS  Google Scholar 

  18. Larson, C.L., Philip, C.B., Wicht, W.C., and Hughes, L.E. (1951)J. Immunol. 67, 289–298.

    PubMed  CAS  Google Scholar 

  19. Saslaw, S., and Carlisle, H.N. (1961)Amer. J. Med. Sci. 242, 166–172.

    PubMed  CAS  Google Scholar 

  20. Hill, L.R. (1966)J. Gen. Microbiol. 44, 419–437.

    PubMed  CAS  Google Scholar 

  21. Ritter, D.B., and Gerloff, R.K. (1966)J. Bacteriol. 92, 1838–1839.

    PubMed  CAS  Google Scholar 

  22. Kates, M. (1964)Adv. Lipid Res. 2, 17–90.

    PubMed  CAS  Google Scholar 

  23. Shaw, N. (1974)Adv. Appl. Microbiol. 17, 63–108.

    Article  PubMed  CAS  Google Scholar 

  24. Abbas, C.A., and Card, G.L. (1980)Biochim. Biophys. Acta 602, 469–476.

    Article  PubMed  CAS  Google Scholar 

  25. Thiele, O.W., and Schwinn, G. (1973)Eur. J. Biochem. 34, 333–344.

    Article  PubMed  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Microbiology and Infectious Diseases, University of Calgary, Health Sciences Centre, T2N 4N1, Calgary, Alberta, Canada

    Robert Anderson

  2. Preventive Medicine Section, Defense Research Establishment Suffield, TOJ 2NO, Ralston, Alberta, Canada

    A. Rashid Bhatti

Authors
  1. Robert Anderson
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  2. A. Rashid Bhatti
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Anderson, R., Rashid Bhatti, A. Fatty acid distribution in the phospholipids ofFrancisella tularensis . Lipids 21, 669–671 (1986). https://doi.org/10.1007/BF02537220

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  • DOI: https://doi.org/10.1007/BF02537220

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