Skip to main content
SpringerLink
Log in

Separation of cholesterol, and fatty acylglycerols, acids and amides by thin-layer chromatography

  • Methods
  • Published:
Lipids

Abstract

A rapid unidimensional thin-layer chromatographic (TLC) method for the separation of neutral lipids is described, using two sequential solvent systems of different polarity. Excellent separations of mono-, di- and triglycerides, fatty acids, fatty amides, and cholesterol are thereby achieved. Separation is accomplished at room temperature and requires 25 min.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

TLC:

thin-layer chromatography

References

  1. Morris, L.J. (1963)Lipid Res. 4, 357–359.

    CAS  Google Scholar 

  2. Mangold, H.K. (1969) inThin-Layer Chromatography (Stahl, E., ed.) pp. 363–421, Springer-Verlag, New York.

    Google Scholar 

  3. Wilgrube, H.J., Erb, W., and Boehler, E. (1973)Fette Seifen Anstrichm. 75, 168–169.

    Article  Google Scholar 

  4. Pernes, J.F., Nurit, Y., and de Heaulme, M. (1980)J. Chromatog. 181, 254–258.

    CAS  Google Scholar 

  5. Bilyk, A. (1981)J. Food. Sci. 46, 298–299.

    Article  CAS  Google Scholar 

  6. Duck-Chong, C.G., and Baker, G.J. (1983)Lipids 18, 387–389.

    PubMed  CAS  Google Scholar 

  7. Kovács, L., Zalka, A., Dobó, R., and Pucsok, J. (1986)J. Chromatog. 382, 308–313.

    Google Scholar 

  8. Bitman, J., Wood, D.L., and Ruth, J.M. (1981)J. Liquid Chromatog. 4, 1007–1021.

    CAS  Google Scholar 

  9. Snyder, F. (1973)J. Chromatog. 82, 7–14.

    Article  CAS  Google Scholar 

  10. Krafft, F., and Stauffer, B., (1882)Ber. Dtsch. Chem. Ges. 15, 1728–1731.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. ARS, Eastern Regional Research Center, U.S. Department of Agriculture, 600 East Mermaid Lane, 19118, Philadelphia, PA

    Alexander Bilyk, George J. Piazza, Raymond G. Bistline Jr. & Michael J. Haas

Authors
  1. Alexander Bilyk
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. George J. Piazza
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Raymond G. Bistline Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Michael J. Haas
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Reference to brand of firm name does not constitute endorsement by the United States Department of Agriculture over others of a similar nature not mentioned.

About this article

Cite this article

Bilyk, A., Piazza, G.J., Bistline, R.G. et al. Separation of cholesterol, and fatty acylglycerols, acids and amides by thin-layer chromatography. Lipids 26, 405–406 (1991). https://doi.org/10.1007/BF02537208

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02537208

Keywords

Search

Navigation