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Comparison of body weight and adipose tissue in male C57BI/6J mice fed diets with and withouttrans fatty acids

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Lipids

Abstract

The effect of a diet containingtrans-fatty acids (tFA) on the fatty acid composition and fat accumulation in adipose tissue was investigated in mice. Male C57BI/6J mice were fed Control or Trans Diets that were similar, except that 50% of the 18∶1, which was allcis in the Control Diet, was replaced bytFA in the Trans Diet. At selected ages, body weight, epididymal fat pad weight, perirenal fat yield, adipose tissue cellularity and fatty acid composition were examined. Over the time period studied (2–24 mon), the proportion of 18∶0 and 16∶0 tended to decrease whilecis-18∶1 levels increased. Compared to the Control Diet, the Trans Diet resulted in adipose tissue lipids with higher percentages of 14∶0 and 18∶2n−6 and lower percentages ofcis-18∶1 and 20∶4n−6. In polar lipids,tFA replaced saturated fatty acids, whereastFA replacedcis-18∶1 in the nonpolar lipids. Body weights at 16 and 24 mon of age and epididymal fat pad weights at 8–24 mon of age were lower in mice fed the Trans Diet as compared to those fed the Control Diet. At the ages studied, the Trans Diet also resulted in lower values for perirenal fat weights, triacylglycerol to polar lipid ratios, and adipose cell size. The data suggest that chronic consumption oftFA affects lipid metabolism and results in decreased fat accumulation in murine adipose tissue.

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Abbreviations

AIN:

American Institute of Nutrition

FAME:

fatty acid methyl esters

GCGLC:

glass capillary gas-liquid chromatography

tFA:

trans-fatty acids

TLC:

thin-layer chromatography

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Author notes

  1. Subodh Atal

    Present address: Laboratory of Cellular Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, 20892, Bethesda, MD

Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Maryland, 20742, College Park, Maryland

    Subodh Atal & Joseph Sampugna

  2. National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 20872, Bethesda, Maryland

    Mary Jane Zamowski & Samuel W. Cushman

Authors
  1. Subodh Atal
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  2. Mary Jane Zamowski
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  3. Samuel W. Cushman
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  4. Joseph Sampugna
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Atal, S., Zamowski, M.J., Cushman, S.W. et al. Comparison of body weight and adipose tissue in male C57BI/6J mice fed diets with and withouttrans fatty acids. Lipids 29, 319–325 (1994). https://doi.org/10.1007/BF02537184

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  • DOI: https://doi.org/10.1007/BF02537184

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