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An improved method for the measurement of malondialdehyde in biological samples

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Lipids

Abstract

An improved method was developed for measuring malondialdehyde (MDA) as its thiobarbituric acid (TBA) complex. Samples were initially incubated with 1% potassium iodide and 0.1% butylated hydroxytoluene at 50°C for 20 min, and then with 0.4% TBA at 60°C for 60 min. The MDA-TBA complex formed was extracted with isobutyl alcohol and measured by high-performance liquid chromatography with fluorescence detection. The improved method allows for a more specific determination of MDA present in biological samples.

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Abbreviations

BHT:

butylated hydroxytoluene

HPLC:

high-performance liquid chromatography

LOH:

hydroxy fatty acid

LOOH:

fatty acid hydroperoxide

MDA:

malondialdehyde

TBA:

thiobarbituric acid

TMP:

1,1,3,3-tetramethoxypropane

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Authors and Affiliations

  1. Department of Nutrition and Food Science, University of Kentucky, 219 Funkhouser Building, 40506-0054, Lexington, KY

    Xiu-Yan Li & Ching K. Chow

Authors
  1. Xiu-Yan Li
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  2. Ching K. Chow
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Li, XY., Chow, C.K. An improved method for the measurement of malondialdehyde in biological samples. Lipids 29, 73–75 (1994). https://doi.org/10.1007/BF02537094

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  • DOI: https://doi.org/10.1007/BF02537094

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