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Effects oftrans fatty acids on lipid accumulation in 3T3-L1 cells

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Lipids

Abstract

Previous work had shown that dietarytrans fatty acids (tFA) resulted in decreased fat deposition in adipose tissue. This study was conducted to see iftFA influence lipid accumulation in Swiss mouse fibroblast 3T3-L1 cells, which are widely used as an adipocyte model. Cells were cultured in the presence of experimental or control growth media supplemented with fatty acids complexed to bovine serum albumin. Fatty acid compositions of experimental and control growth media were similar except that the octadecenoates in the control growth media werecis fatty acids, whereas those in the experimental media contained bothcis andtrans fatty acids. Cell-conditioned media and cellular lipids at the preadipocyte and differentiating adipocyte stages were analyzed. At both stages of development, less fat accumulated, in cells cultured in the presence oftFA, due primarily to a decrease in the nonpolar lipid content of cells exposed totFA, and linoleate to arachidonate ratios were higher in cells supplemented withtFA. Calculations comparing sums of saturated and monounsaturated fatty acids in cells at the differentiating adipocyte stage suggested thattFA may have replaced monoun-saturated fatty acids in the nonpolar lipid fraction and saturated fatty acids in the polar lipid fraction. The results of these studies are in good agreement with thein vivo effects oftFA seen in previous work with mouse adipose tissue. It was concluded that the 3T3-L1in vitro model is an appropriate system for further studies oftFA and lipid metabolism in adipose tissue.

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Abbreviations

BSA:

bovine serum albumin

cAMP:

3′, 5′-cyclic adenosine monophosphate

CGM:

control growth medium

DA:

differentiating adipocytes

DEX:

dexamethasone

DMEM:

Dulbecco's modified Eagle medium

EGM:

experimental growth medium

FAME:

fatty acid methyl esters

FCS:

fetal calf serum

GM:

growth medium

HDL:

high density lipoprotein

LDL:

low density lipoprotein

LPSR-1:

low protein serum replacement

MIX:

3-isobutyl-1-methyl xanthine

NEAA:

nonessential amino acids

PA:

preadipocytes

PBS:

phosphate buffered saline

tFA:

trans fatty acids

TLC:

thin-layer chromatography

3T3-L1:

subclone of Swiss mouse embryo 3T3 fibroblasts

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Maryland, 20742, College Park, Maryland

    Kalpana Panigrahi & Joseph Sampugna

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  1. Kalpana Panigrahi
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  2. Joseph Sampugna
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Panigrahi, K., Sampugna, J. Effects oftrans fatty acids on lipid accumulation in 3T3-L1 cells. Lipids 28, 1069–1074 (1993). https://doi.org/10.1007/BF02537072

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  • DOI: https://doi.org/10.1007/BF02537072

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