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Thermal acclimation and dietary lipids alter the composition, but not fluidity, of trout sperm plasma membrane

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Lipids

Abstract

The effect of a long-term adaptation of rainbow trout to 8 and 18°C combined with a corn oil-or a fish oil-supplemented diet on the characteristics of the spermatozoan plasma membrane was investigated. The experiment lasted up to 22 mon during which spermatozoa were collected from the mature males. Spermatozoan plasma membranes were isolated by nitrogen cavitation, and the cholesterol content, phospholipid composition and fatty acid pattern were investigated. Membrane viscosity was assessed on whole cells by electron spin resonance using spin-labeled phospholipids. Neither diet nor rearing temperature influenced the cholesterol content of the plasma membrane nor the phospholipid class distribution. The rearing temperature of the broodstock only slightly affected the phospholipid fatty acids. A minor decrease in 18∶0 and increase in monounsaturated fatty acids was observed for the cold-adapted fish. These modifications were not sufficient to affect membrane fluidity, and we conclude that trout spermatozoa do not display any homeoviscous adaptations in these conditions. On the contrary, the dietary fatty acid intake greatly modified the fatty acid profile of plasma membrane phospholipids. The fish oil-fed trout displayed a much higher n−3/n−6 fatty acid ratio than did the corn oil-fed ones, but the 22∶6n−3 levels remained unchanged. Modifications in plasma membrane composition by the diet were obtained although neither of the two diets was deficient in essential fatty acids. The enrichment in n−3 fatty acids, however, did not affect plasma membrane fluidity which was unchanged by the diets.

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Abbreviations

BCA:

bicinchoninic acid

BHT:

butylated hydroxytoluene

DFP:

diisopropyl fluorophosphate

EFA:

essential fatty acid

ESR:

electron spin resonance

HPLC:

high-performance liquid chromatography

MUFA:

monounsaturated fatty acid

SE:

standard error

SL-PC:

spin-labeled phosphatidylcholine

SL-PS:

spin-labeled phosphatidylserine

PUFA:

polyunsaturated fatty acid

SFA:

saturated fatty acid

UFA:

unsaturated fatty acid

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Author notes

  1. K. Müller

    Present address: Institut für Fortpflanzung Landwirtschaftlicher Nutztiere, Schönow e.V., 16321, Schönow, Germany

Authors and Affiliations

  1. Institut de Biologie Physico-Chimique, Paris, France

    K. Müller & A. Zachowski

  2. Laboratoire de Nutrition des Poissons, INRA, Saint Pée sur Nivelle, France

    S. Kaushik

  3. Laboratoire de Physiologie des Poissons, INRA, Campus de Beaulieu, 35042, Rennes Cedex, France

    C. Labbe, G. Maisse & M. Loir

Authors
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Labbe, C., Maisse, G., Müller, K. et al. Thermal acclimation and dietary lipids alter the composition, but not fluidity, of trout sperm plasma membrane. Lipids 30, 23–33 (1995). https://doi.org/10.1007/BF02537038

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  • DOI: https://doi.org/10.1007/BF02537038

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