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Plasma and lipoprotein lipid peroxidation in humans on sunflower and rapessed oil diets

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Lipids

Abstract

The effects of natural mixed diets on lipid peroxidation were investigated in humans. In the first study, 59 subjects were fed a rapeseed oil-based diet rich in monounsaturated fatty acids (MUFA) and a sunflower oil-based diet rich in polyunsaturated fatty acids (PUFA) in a cross-over manner for three and a half weeks. The lipid peroxidation products in plasma were determined by measuring conjugated dienes and malondialdehyde (MDA). In a second study, plasma thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides, and the susceptibility of very low density lipoprotein + low-density lipoprotein (LDL) toin vitro oxidation were measured from subjects fed similar MUFA and PUFA diets for six week diets. No significant differences in plasma MDA or conjugated diene concentrations were found after the rapeseed oil diet or the sunflower oil diet in Study 1. In the second study, a small but significant decrease (P<0.05) in both lipid hydroperoxides and TBARS was observed in the LDL fraction after the sunflower oil diet. Thein vitro oxidation gave opposite results, showing increased oxidation after the sunflower oil diet. Despite a high intake of α-tocopherol during the oil peroids, no increase in plasma α-tocopherol was noticed in either study. The results suggest that moderate changes in the fatty acid composition in the Western-type diet may be adequate to affect lipoprotein susceptibility to oxidationin vitro, but there is considerable disparity with some indices ofin vivo lipid peroxidation.

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Abbreviations

BHT:

butylated hydroxytoluene

BuOH:

n-buthanol

CV:

coefficient of variation

en%:

percent of energy

GSHPx:

glutathione peroxidase

HDL:

high-density lipoprotein

HPLC:

high-performance liquid chromatography

IDL:

intermediate-density lipoprotein

LDL:

low-density lipoprotein

MDA:

malondialdehyde

MUFA:

monounsaturated fatty acids

PBS:

phosphate buffered saline

PUFA:

polyunsaturated fatty acids

TBA:

thiobarbituric acid

TBARS:

thiobarbituric acid reactive substances

VLDL:

very low density lipoprotein

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Author information

Author notes

  1. Liisa Valsta

    Present address: Department of Nutrition, National Public Health Institute, SF-00300, Helsinki, Finland

  2. Eino Hietanen

    Present address: Department of Clinical Physiology, University of Turku, SF-20520, Turku, Finland

Authors and Affiliations

  1. Department of Applied Chemistry and Microbiology, Division of Nutrition, University of Helsinki, P.O. Box 27, 00014, Finland

    Anu M. Turpeinen, Liisa Valsta & Marja Mutanen

  2. Department of Nutrition, National Public Health Institute, SF-00300, Helsinki, Finland

    Georg Alfthan

  3. International Agency for Research on Cancer, Lyon, F-69372, Lyon, France

    Eino Hietanen & Hanna Schunk

  4. Research Institute of Public Health, University of Kuopio, SF-70211, Kuopio, Finland

    Jukka T. Salonen & Kristiina Nyyssönen

Authors
  1. Anu M. Turpeinen
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  2. Georg Alfthan
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  3. Liisa Valsta
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Turpeinen, A.M., Alfthan, G., Valsta, L. et al. Plasma and lipoprotein lipid peroxidation in humans on sunflower and rapessed oil diets. Lipids 30, 485–492 (1995). https://doi.org/10.1007/BF02537021

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  • DOI: https://doi.org/10.1007/BF02537021

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