Skip to main content
SpringerLink
Log in

Platelet-activating factor (PAF) in allergic diseases: Inhibitory effects of anti-allergic drugs, ketotifen and three Kampo medicines on PAF production

  • PAF, the Respiratory System, Allergy, and Asthma
  • Published:
Lipids

Abstract

Platelet-activating factor (PAF) is considered an important mediator in allergic and inflammatory reactions. To further investigate the role of PAF in allergic diseases, we examined thein vitro andex vivo effect of anti-allergic drugs on PAF production by human neutrophils. Ketotifen and three Kampo medicines, which are widely used in the treatment of allergic diseases in Japan, inhibited PAF production by normal human neutrophils dose-dependently, whereas disodium chromoglycate (DSCG) and tranilast did not have any inhibitory effect. Ketotifen also suppressedex vivo PAF production by neutrophils from healthy subjects. PAF production decreased from 40.5±5.0 units to 21.6±4.7 units after oral administration of 2 mg of ketotifen per day for one week. We also measured the concentration of lysoPAF in serum from patients with Japanese cedar pollinosis during the pollen season and followed the effect of ketotifen on lysoPAF levels in the serum from these patients. We found that the concentration of lysoPAF, a precursor and degradation product of PAF, in patients with cedar pollinosis (87.8±8.7 units) was significantly higher than in healthy subjects (54.7±7.7 units). After two weeks, lysoPAF levels decreased significantly (82.6 units to 41.3 units) only in the group treated with nasal aerosol and ketotifen. These results suggest that PAF may play a role in allergic diseases and that the clinical effects of anti-allergic drugs may at least partially be due to their anti-PAF action.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BSA:

bovine serum albumin

DSCG:

disodium chromoglycate

lysoPAF:

lyso platelet-activating factor, 1-O-alkyl-sn-glycero-3-phosphocholine

PAF:

1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine

PBS:

phosphate-buffered saline

References

  1. O'Flaherty, J.T., and Wykle, R.T. (1983)Clin. Rev. Allergy 1, 353–367.

    PubMed  Google Scholar 

  2. Morley, J., Sanjar, S., and Page, C.P. (1984)Lancet ii, 1142–1144.

    Article  Google Scholar 

  3. Page, C.P., Archer, C.B., Paul, W., and Morley, J. (1984)TIPS III, 239–241.

    Google Scholar 

  4. Patterson, R., and Harris, K.E. (1983)J. Lab. Clin. Med. 102, 933–938.

    PubMed  CAS  Google Scholar 

  5. Vargaftig, B.B., Lefort, J., Chignard, M., and Benveniste, J. (1980)Eur. J. Pharmacol. 65, 185–192.

    Article  PubMed  CAS  Google Scholar 

  6. Nakamura, T., Morita, Y., Kuriyama, M., Ishihara, K., Ito, K., and Miyamoto, T. (1987)Int. Archs. Allergy Appl. Immun. 82, 57–61.

    CAS  Google Scholar 

  7. Horiguchi, S., and Saito, Y. (1964)Jap. J. Allegol. 13, 19–24.

    Google Scholar 

  8. Ishizaki, T., Koizumi, K., Ikemori, R., Ishiyama, Y., and Kushibiki, E. (1987)Ann. Allergy 58, 265–270.

    PubMed  CAS  Google Scholar 

  9. Satouchi, K., Oda, M., Saito, K., and Hanahan, J.D. (1984)Arch. Biochem. Biophys. 234, 318–321.

    Article  PubMed  CAS  Google Scholar 

  10. Satouchi, K., Oda, M., Yasunaga, K., and Saito, K. (1983)J. Biochem. 94, 2067–2070.

    PubMed  CAS  Google Scholar 

  11. Bligh, E.G., and Dyer, W. (1959)Can. J. Biochem. Physiol. 37, 911–917.

    PubMed  CAS  Google Scholar 

  12. Terashita, Z., Tsushima, S., Yoshoka, Y., Nomura, H., Inada, Y., and Nishikawa, K. (1983)Life Science 32, 1975–1982.

    Article  CAS  Google Scholar 

  13. Snyder, F. (1985)Med. Res. Rev. 5, 107–140.

    Article  PubMed  CAS  Google Scholar 

  14. Tan, E.L., and Snyder, F. (1985)Thromb. Res. 38, 713–717.

    Article  PubMed  CAS  Google Scholar 

  15. Robinson, M., and Snyder, F. (1985)Biochim. Biophys. Acta 837, 52–56.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Internal Medicine and Physical Therapy, School of Medicine, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, Japan

    Tsuneyoshi Nakamura, Motoaki Kuriyama, Keiko Ishihara & Terumasa Miyamoto

  2. Matsumura Clinic and Research Institute for Asthma and Immunological Diseases, 5-52-2, Higashinippori, Arakawa-ku, Tokyo, Japan

    Yukio Matsumura

Authors
  1. Tsuneyoshi Nakamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Motoaki Kuriyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Keiko Ishihara
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yukio Matsumura
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Terumasa Miyamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Nakamura, T., Kuriyama, M., Ishihara, K. et al. Platelet-activating factor (PAF) in allergic diseases: Inhibitory effects of anti-allergic drugs, ketotifen and three Kampo medicines on PAF production. Lipids 26, 1297–1300 (1991). https://doi.org/10.1007/BF02536552

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02536552

Keywords

Search

Navigation