Abstract
It recently has been recognized that platelet-activating factor (PAF) may be a mediator of asthma exacerbation. We had the opportunity to analyze bronchoalveolar lavage fluids from an asthmatic infant, which were characterized by neutrophil infiltration. The patient's lungs were washed on three occasions with saline during asthmatic attacks. PAF was found in each case on the basis of its ability to cause the immediate aggregation of washed rabbit platelets. The PAF detected was equivalent to 1–1.4 pmol of 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine, three quarters of which were recovered in cell-associated form. By contrast, we did not detect PAF in bronchoalveolar exudates from patients with larungeal stenosis or with respiratory distress syndrome. LysoPAF, the direct precursor as well as initial metabolite of PAF, was also analyzed after being converted to PAF by acetylation. There was a wide variation in the amount of lysoPAF present in individual patients, suggesting that lysoPAF levels cannot be taken as an indicator for the presence of PAF.
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Abbreviations
- BALP:
-
bronchoalveolar lavage fluid
- BSA:
-
bovine serum albumin
- IoA23187:
-
Calcium Ionophore A23187
- fMLP:
-
formyl-methionyl-leucyl-phenylalanine
- GPC:
-
sn-glycero-3-phosphocholine
- PAF:
-
platelet-activating factor
- PC:
-
phosphatidylcholine
- RDS:
-
respiratory distress syndrome
- SIM:
-
selected ion monitoring
- t-BDMS:
-
tert-butyldimethylsilyl
- TLC:
-
thin-layer chromatography
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Horii, T., Okazaki, H., Kino, M. et al. Platelet-activating factor detected in bronchoalveolar lavage fluids from an asthmatic patient. Lipids 26, 1292–1296 (1991). https://doi.org/10.1007/BF02536551
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DOI: https://doi.org/10.1007/BF02536551