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Increased amounts of cholesterol precursors in lipoproteins after ileal exclusion

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Lipids

Abstract

Serum cholesterol precursor sterols reflect the activity of cholesterol synthesis. In this study, squalene, methyl sterol and lathosterol contents were studied in very low density lipoprotein (VLDL), low density lipoprotein (LDL) and high density lipoprotein (HDL) of heterozygous familial hypercholesterolemia patients without and with ileal bypass. The contents of lathosterol and all methyl sterols (lanosterol, Δ8,24-dimethylsterol, Δ8-dimetylsterol, Δ8-methostenol and methostenol), but not of squalene were increased in all lipoproteins by ileal bypass. The increase in the free methyl sterols was more marked than that in the esterified ones. The percentage esterification of the methyl sterols was highest in HDL and lowest in VLDL. Lipoprotein methyl sterol contents were positively correlated with each other and with cholesterol synthesis. The methyl sterols were slightly concentrated in LDL, and squalene strongly concentrated in VLDL. It is concluded that long-term stimulation of cholesterol synthesis increases the methyl sterols in all lipoproteins.

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Abbreviations

Cholesterol:

cholest-5-en-3β-ol

Δ8-dimethylsterol (4,4-dimethyl-5α-cholest-8-en-3β-ol):

4,4-dimethyl-5α-cholest-8-en-3β-ol

Δ8,24-dimethylsterol (4,4-dimethyl-5α-cholest-8(7),24-dien-3β-ol):

4,4-dimethyl-5α-cholest-8(7), 24-dien-3β-ol

HDL:

high density lipoprotein

HMG-CoA:

hydroxymethylglutaryl coenzyme A

lanosterol:

4,4,14α-trimethyl-5α-cholest-8,24-dien-3β-ol

lathosterol:

5α-cholest-7-en-3β-ol

LDL:

low density lipoprotein

methostenol:

4α-methyl-5α-cholest-7-en-3β-ol

Δ8-methostenol (4α-methyl-5α-cholest-8-en-3β-ol):

4α-methyl-5α-cholest-8-en-3β-ol

VLDL:

very low density lipoprotein

GLC:

gas liquid chromatography

TLC:

thin layer chromatography

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Authors and Affiliations

  1. Second Department of Medicine, University of Helsinki, SF-OO29O, Helsinki, Finland

    Pekka V. I. Koivisto & Tatu A. Miettinen

Authors
  1. Pekka V. I. Koivisto
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  2. Tatu A. Miettinen
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Koivisto, P.V.I., Miettinen, T.A. Increased amounts of cholesterol precursors in lipoproteins after ileal exclusion. Lipids 23, 993–996 (1988). https://doi.org/10.1007/BF02536349

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  • DOI: https://doi.org/10.1007/BF02536349

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