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The effect of two synthetic phospholipids on cell proliferation and phosphatidylcholine biosynthesis in Madin-Darby canine kidney cells

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Lipids

An Erratum to this article was published on 01 July 1995

Abstract

The concentration-dependent effects of two different synthetic phospholipids on cell proliferation and phosphatidylcholine biosynthesis were compared in Madin-Darby canine kidney (MDCK) cells. The alkyllysophospholipid 1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine and the alkylphosphocholine, hexadecylphosphocholine, inhibited cell proliferation with half-inhibitory concentrations (IC50) of 75 and 135 μmol/L, respectively. The agents also inhibited phosphatidylcholine biosynthesis in confluent and proliferating MDCK cells. The IC50 of 1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine was 40 μmol/L in confluent cells and 20 μmol/L in proliferating cells, whereas the IC50 of hexadecylphosphocholine was higher in both experimental systems (67 μmol/L in confluent cells and 40 μmol/L in proliferating cells). Further experiments revealed that the effect of both agents on phosphatidylcholine biosynthesis was reversible and that the inhibition was mediated by translocation of the rate-limiting enzyme of this pathway, CTP: phosphocholine cytidylyltransferase (EC 2.7.7.15), from membranes to the cytosol, where it is inactive. The present findings suggest that the inhibition of phosphatidylcholine biosynthesis by both synthetic phospholipids might be related, in part, to their antiproliferative effects.

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Abbreviations

BCA:

bicinchoninic acid

ET-18 OCH3 :

1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine

HePC:

hexadecylphosphocholine

HPTLC:

high-performance thin-layer chromatography

IC50 :

half-inhibitory concentration

MDCK:

Madin-Darby canine kidney

PBS:

phosphate-buffered saline

TPA:

12-O-tetradecanoylphorbol-13-acetate

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Authors and Affiliations

  1. Haut- und Poliklinik, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Hindenburgdamm 30, 12200, Berlin, Germany

    Thomas Wieder, Anne Haase, Christoph C. Geilen & Constantin E. Orfanos

Authors
  1. Thomas Wieder
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  2. Anne Haase
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  3. Christoph C. Geilen
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  4. Constantin E. Orfanos
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Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/BF02537007.

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Wieder, T., Haase, A., Geilen, C.C. et al. The effect of two synthetic phospholipids on cell proliferation and phosphatidylcholine biosynthesis in Madin-Darby canine kidney cells. Lipids 30, 389–393 (1995). https://doi.org/10.1007/BF02536296

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  • DOI: https://doi.org/10.1007/BF02536296

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