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Effects of polyphenolic natural products on the lipid profiles of rats fed high fat diets

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Lipids

Abstract

Male Wistar rats were fed a high fat diet (HFD) containing 2.5% cholesterol and 16% lard supplemented with polyphenolic natural products namely quercetin, morin or tannic acid (100 mg/rat/day) for 4, 7 and 10 wk. Rats fed HFD without the supplements served as control. The effects of these compounds on blood lipid profiles, enzymes, liver fat and aorta of the rat were studied. In rats fed HFD containing tannic acid, plasma total cholesterol (TC), low density lipoprotein cholesterol (LDLC) and triglyceride (TG) were reduced by 33.3%, 29.6% and 65.1%, respectively, at week 10. High density lipoprotein cholesterol (HDLC) concentration was not altered. Fat deposition was also decreased in the liver of these rats. Morin significantly reduced plasma TG (65.1%) and liver fat only at week 7 while at week 10 it reduced plasma TC and LDLC by 30.9% and 29.3% respectively. The plasma HDLC concentration was increased by 47.3% at week 4 but no effect was seen at weeks 7 and 10. In the rats fed HFD containing quercetin, plasma HDLC was increased by 28.6% at week 7 but at week 10, plasma LDLC was increased by 21.2%. Quercetin did not cause any significant changes on the plasma TC, TG and liver fat at weeks 4, 7 and 10. Plasma alanine aminotransferase, alkaline phosphatase and bilirubin in control and treated groups were not significantly different. However, hepatic lipase activity in rats fed tannic acid was significantly lower. Aortae of all groups of rats showed no abnormalities. The present report indicates that tannic acid and morin are effective in reducing plasma and liver lipids when supplemented with a high fat diet in rats.

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Abbreviations

ALAT:

alanine aminotransferase

ANOVA:

analysis of variance

AP:

alkaline phosphatase

ATP:

adenosine triphosphate

BF3 :

borontrifluoride

BHT:

butylated hydroxytoluene

CHD:

coronary heart disease

EDTA:

ethylenediaminetetraacetate

G1:

group 1

G2:

group 2

G3:

group 3

G4:

group 4

G5:

group 5

HDLC:

high density lipoprotein cholesterol

HFD:

high fat diet

HFD+M:

high fat diet + morin

HFD+T:

high fat diet + tannic acid

HFD+Q:

high fat diet + quercetin

LDLC:

low density lipoprotein cholesterol

NADH:

reduced nicotinamide adenine dinucleotide

ND:

normal diet

TC:

total cholesterol

TG:

triglyceride

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, National University of Singapore, 0511, Singapore, Republic of Singapore

    T. Yugarani & N. P. Das

  2. Department of Pharmacology, Faculty of Medicine, National University of Singapore, 0511, Singapore, Republic of Singapore

    B. K. H. Tan

  3. Department of Pathology, Faculty of Medicine, National University of Singapore, 0511, Singapore, Republic of Singapore

    M. Teh

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  1. T. Yugarani
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  3. M. Teh
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  4. N. P. Das
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Yugarani, T., Tan, B.K.H., Teh, M. et al. Effects of polyphenolic natural products on the lipid profiles of rats fed high fat diets. Lipids 27, 181–186 (1992). https://doi.org/10.1007/BF02536175

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