Central nervous system demyelinating diseases and increased release of cholesterol into the urinary system of rats

Abstract

The question of what happens to cholesterol in the adult central nervous system during its slow turnover has been addressed using rats with brain and spinal cord labeled with [4-¹⁴C]cholesterol upon intracerebral injection of labeled cholesterol into rats at 10–12 days of age. At six months after injection,¹⁴C was found only in the brain and spinal cord and was slowly releasedvia the rat's urine. When labeled rats were given demyelinating agents (triethyl tin chloride, hexachlorophene, sodium cyanide) and when experimental allergic encephalomyelitis was induced, a measurable increase in urinary¹⁴C label above control levels was found. It was concluded that there is a direct relationship between the experimental demyelination induced and the increased release of cholesterol metabolites into urine. The study suggests that a clinical method could be developed to determine the rate of central nervous system demyelination by measuring the amount of urinary cholesterol metabolites.