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Investigations on the cellular uptake of hexadecylphosphocholine

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Lipids

Abstract

The uptake of [(9,10)-3H]hexadecylphosphocholine (HePC) in six tumor cell lines was studied. All cell lines incorporated HePC in similar amounts, with the exception of the epidermoid cancer cell line KB, which took up higher amounts of HePC. The uptake of HePC at 37°C was shown to be time and concentration dependent. At 20°C, uptake was drastically reduced and at 4°C it was blocked completely. Binding of HePC, at 4°C, was not saturable at concentrations between 5 βg/mL (11.8 μM) and 100 μg/mL (235.3 μM), indicating that cell surface binding is not receptor-mediated. Furthermore, the effects of inhibitors of endocytosis were investigated. We observed a pronounced inhibitory effect by monensin and cytochalasin B. Colchicine was somewhat less effective whereas chloroquine was almost without effect. From these data we conclude that uptake of HePC is most probably mediatedvia a receptor-independent endocytotic mechanism.

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Abbreviations

ATP:

adenosine triphosphate

FCS:

fetal calf serum

HePC:

hexadecylphosphocholine

NaSCN:

sodium thiocyanate

NIM:

neutrophile isolation medium

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Authors and Affiliations

  1. Max-Planck-Institute for Biophysical Chemistry, 37077, Goettingen, Germany

    Eduard A. M. Fleer & Hansjoerg Eibl

  2. Department of Hematology and Oncology, University Clinic of Goettingen, Robert-Koch-Strasse 40, 37077, Goettingen, Germany

    Eduard A. M. Fleer, Dinko Berkovic & Clemens Unger

Authors
  1. Eduard A. M. Fleer
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  2. Dinko Berkovic
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  3. Hansjoerg Eibl
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  4. Clemens Unger
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Fleer, E.A.M., Berkovic, D., Eibl, H. et al. Investigations on the cellular uptake of hexadecylphosphocholine. Lipids 28, 731–736 (1993). https://doi.org/10.1007/BF02535995

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  • DOI: https://doi.org/10.1007/BF02535995

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