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Specific accumulation of docosahexaenoic acid (22∶6n−3) in brain lipids during development of juvenile turbotScophthalmus maximus L.

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Lipids

Abstract

The changes in the lipid class and the fatty acid compositions of total lipids and individual glycerophospholipids which occur in brain during development of juvenile turbot (Scophthalmus maximus L.) were investigated. Fish were sampled during a 10-week period immediately following weaning from a live feed to a pellet diet. During this period, brain dry weight increased over 6-fold. The percentages of protein, cholesterol and galactolipids increased in brain during development, presumably reflecting increased membrane maturation and myelination processes. The percentages of docosahexaenoic acid (DHA; 22∶6n−3) were low at the beginning of the study period. However, DHA specifically accumulated in juvenile turbot brain during development. The percentages of DHA increased in total lipid, total diradyl glycerophosphocholine (GPC), total diradyl glycerophosphoethanolamine (GPE), phosphatidylserine (PS) and phosphatidylinositol (PI) reaching 26.1%, 25.8%, 40.8%, 47.1% and 17.9% of the total fatty acids, respectively, by the end of the 10-week period. The percentages of other n−3 polyunsaturated fatty acids (PUFA) and n−6 PUFA generally decreased during this period, as did that of monoenes; the percentages of saturated fatty acids remained relatively constant. Non-linear regression analysis showed that the increase in DHA in total lipid, GPC, GPE and PS fitted 1st order rate kinetics (plus offset) allowing maximum values for the percentages of DHA in each lipid class to be estimated.

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Abbreviations

BHT:

butylated hydroxytoluene

CL:

cardiolipin

DH:

docosahexaenoic acid

DMA:

dimethyl acetal

EPA:

eicosapentaenoic acid

GPC:

total diradyl glycerophosphoethanolamine

GPE:

total diradyl glycerophosphocholine

HPLC:

high-performance liquid chromatography

HPTLC:

high-performance thin-layer chromatography

HUFA:

highly unsaturated fatty acid (≥C20 and with at least 3 double bonds)

PA:

phosphatidic acid

PI:

(diacyl)phosphatidylinositol

PS:

(diacyl)phosphatidylserine

PUFA:

polyunsaturated fatty acid

TLC:

thin-layer chromatography

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Author notes

  1. Gabriel Mourente

    Present address: Departmento de Biología, Facultad de Ciencias del Mar, Universidad de Cádiz. Poligono Rio San Pedro, 11510, Puerto Real (Cádiz), Spain

Authors and Affiliations

  1. NERC Unit of Aquatic Blochemistry, Department of Molecular and Biological Sciences, School of Natural Sciences, University of Stirling, FK9 4LA, Stirting, Scotland

    Gabriel Mourente, Douglas R. Tocher & John R. Sargent

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  1. Gabriel Mourente
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  2. Douglas R. Tocher
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  3. John R. Sargent
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Mourente, G., Tocher, D.R. & Sargent, J.R. Specific accumulation of docosahexaenoic acid (22∶6n−3) in brain lipids during development of juvenile turbotScophthalmus maximus L.. Lipids 26, 871–877 (1991). https://doi.org/10.1007/BF02535970

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